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3.
Fig. 6

Fig. 6. Combined effects of fibrin and growth factors on MSC phenotypic markers after 12 days. From: Biophysical and Chemical Effects of Fibrin on Mesenchymal Stromal Cell Gene Expression.

Real-time PCR data shows the differential gene expression for vasculogenic markers CNN1 (A) and VECAD (B); myogenic markers MHF5 (C) and MYH13 (D); neurogenic marker GFAP (E), and osteogenic marker BMP2 (F). The data is expressed as normalized raw abundance. Con represents control tissue culture substrate and FG represents fibrin-coated substrates. # indicates statistically significant compared to growth factor treatment without fibrin. Statistically significant relationships are denoted by *P<0.05, **P<0.01, and ***P<0.001 (n≥3).

Ngan F. Huang, et al. Acta Biomater. ;6(10):3947-3956.
4.
Fig 1

Fig 1. Mesenchymal stem cell (MSC) phenotype on fibrin-coated surfaces. From: Biophysical and Chemical Effects of Fibrin on Mesenchymal Stromal Cell Gene Expression.

Real-time PCR data shows the differential gene expression after 5 days, as quantified as normalized mRNA quantity, for phenotypic markers comprising vasculogenic (A–B), myogenic (C–D), neurogenic (E–F), chondrogenic (G), osteogenic (G), and adipogenic (G) lineages. Immunoblots depicting FLK1, ACTA2, MYF5, and total actin protein expression (H) are shown and quantified by densitometry (I–K). Error bars indicate standard deviation. Con represents control tissue culture substrate and FG represents fibrin-coated substrates. * P<0.05, **P<0.01, ***P<0.001 (n≥3).

Ngan F. Huang, et al. Acta Biomater. ;6(10):3947-3956.
5.
Fig. 4

Fig. 4. Effect of ECM on MSC differentiation markers after 2 days. From: Biophysical and Chemical Effects of Fibrin on Mesenchymal Stromal Cell Gene Expression.

Real-time PCR data shows the differential gene expression for phenotypic markers when MSCs were cultured on laminin (Lam), fibronectin (Fib), fibrin (FG), collagen monomer (Coll Mon), or collagen polymer (Coll Pol). The phenotypic markers consisted of those comprising vasculogenic (A–C), myogenic (D), neurogenic (E), chondrogenic (F), and osteogenic (G) lineages. The PCR data is expressed as a normalized abundance to the control tissue culture substrate (Con) for markers comprising multiple lineages. Error bars represent standard deviation.). * P<0.05, **P<0.01, ***P<0.001 when compared to Con group (n≥3).

Ngan F. Huang, et al. Acta Biomater. ;6(10):3947-3956.
6.
Fig. 2

Fig. 2. Effects of physical and mechanical properties of fibrin on gene expression changes in MSCs. From: Biophysical and Chemical Effects of Fibrin on Mesenchymal Stromal Cell Gene Expression.

A. H&E-stained cross sections of fibrin in the standard formulation (FG), or when thrombin was further diluted 10-fold (T10) or 50-fold (T50), respectively. T10F10 represents fibrin with 10-fold dilution of both thrombin and fibrinogen. Quantification of (B) the average equivalent pore diameter and (C) Young’s modulus for fibrin compositions. D. Real-time PCR data shows the differential gene expression after 2 days of incubation on various fibrin compositions. The PCR data is expressed as normalized mRNA quantities, and error bars indicate standard deviation (n≥3). * P<0.05, **P<0.01, ***P<0.001 (n≥3). Scale bar: 500 μm.

Ngan F. Huang, et al. Acta Biomater. ;6(10):3947-3956.

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