Results: 5

1.
Fig. 2

Fig. 2. From: Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent.

Long-term integrity of capsids and dimers. MS of the 15N-Cp149d (a) and Cp149c (b) controls at 0 and 89 days of incubation at 4°C shows that the proteins were stable. The 15N-Cp149d did not assemble into capsids, and Cp149c did not dissociate to dimers.

Charlotte Uetrecht, et al. Phys Chem Chem Phys. ;12(41):13368-13371.
2.
Fig. 4

Fig. 4. From: Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent.

Exchange rates are largely unaffected by change in pH. Dimer exchange was monitored in samples taken from incubations with pH values ranging from 5.5 to 10.3, for both T = 3 (a) and T = 4 Cp149c (b). Significant exchange occurred only in the T = 3 form, but there was no clear pH dependency for either geometry.

Charlotte Uetrecht, et al. Phys Chem Chem Phys. ;12(41):13368-13371.
3.
Fig. 1

Fig. 1. From: Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent.

Monitoring HBV capsid breathing by MS/MS. 15N-Cp149d was mixed with Cp149c and then analyzed at intervals for up to 90 days by MS/MS. Precursor ions of T = 3 and T = 4 Cp149c were selected (represented by vertical tan-colored bands) and individually subjected to CID. The relative amount of 15N-Cp149d incorporated into the intact Cp149c was determined from the ion intensities of the ejected monomers in the MS/MS spectrum, as shown for the T = 3 Cp149c. Monomer ratios were obtained based on the summed intensities of the different charge states.

Charlotte Uetrecht, et al. Phys Chem Chem Phys. ;12(41):13368-13371.
4.
Fig. 5

Fig. 5. From: Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent.

Surface lattice of T = 3 and T = 4 HBV capsids. Shown is the arrangement of dimers in the T = 3 (a) and T = 4 (b) icosahedral geometries. The quasi-equivalent sites are labelled in both cases (T = 3; A, B, C and T = 4; A, B, C, D). Note that sites with the same symbol do not have identical structures. The 5-, 3-, and 2-fold axes of symmetry are also indicated. In the T = 3 lattice, the red C dimer lies on the two-fold axis of symmetry, and across two facets of the icosahedron. No such environment exists in the T = 4 geometry. Figure reproduced from ref. 3.

Charlotte Uetrecht, et al. Phys Chem Chem Phys. ;12(41):13368-13371.
5.
Fig. 3

Fig. 3. From: Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent.

Subunit exchange depends on capsid geometry and temperature. Dimer exchange was monitored by MS/MS at 4°C, 23°C, and 37°C for both T = 3 (a) and T = 4 (b) Cp149c for up to 120 days. No significant exchange was observed at 23°C (orange) and 37°C (purple) for either geometry. At 4°C, the T = 3 Cp149c exchanged Cp149d with free ones in solution (pink), an exponential fit is shown indicating an endpoint of approximately 13%. T = 4 Cp149c do not show significant levels of exchange at the same temperature (blue). Error bars correspond to the standard deviation of triplicate analysis. The dashed line represents the 100% level.

Charlotte Uetrecht, et al. Phys Chem Chem Phys. ;12(41):13368-13371.

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