Results: 4

1.
Figure 3

Figure 3. From: MicroRNA mir-16 is anti-proliferative in enterocytes and exhibits diurnal rhythmicity in intestinal crypts.

Temporal pattern of cell cycle protein expression in rat intestine. Whole-cell protein lysates of rat intestinal mucosa, harvested at the indicated times, were analyzed by immunoblotting. Diurnal amplitudes and p-values are summarised in Table 2.

Anita Balakrishnan, et al. Exp Cell Res. ;316(20):3512-3521.
2.
Figure 4

Figure 4. From: MicroRNA mir-16 is anti-proliferative in enterocytes and exhibits diurnal rhythmicity in intestinal crypts.

Temporal pattern of mRNA levels for cell cycle genes in rat intestine. Total RNA was extracted from rat intestinal mucosa, harvested at the indicated times, were analyzed by real-time PCR. Diurnal amplitudes and p-values are summarised in Table 2.

Anita Balakrishnan, et al. Exp Cell Res. ;316(20):3512-3521.
3.
Figure 1

Figure 1. From: MicroRNA mir-16 is anti-proliferative in enterocytes and exhibits diurnal rhythmicity in intestinal crypts.

Temporal pattern of microRNAs showing a 2-fold or greater change between any two timepoints (Fig 1A–C). Total RNA was extracted from the intestinal mucosa of rats harvested at the indicated times, run on microRNA microarrays and microRNAs showing a 2-fold or greater change between any two timepoints validated by real-time PCR. mir-16 expression in enterocyte fractions (Fig 1D). Laser capture microdissection and real-time PCR were used to determine mir-16 expression in fractions of crypt, villus and smooth muscle in cryofixed sections of jejunum.

Anita Balakrishnan, et al. Exp Cell Res. ;316(20):3512-3521.
4.
Figure 2

Figure 2. From: MicroRNA mir-16 is anti-proliferative in enterocytes and exhibits diurnal rhythmicity in intestinal crypts.

Effects of mir-16 on enterocyte phenotype in vitro. mir-16 (or a scrambled control) was stably overexpressed in IEC-6 cells as described in Materials and Methods. All assays were performed 48 hours after plating. mir-16 expression was quantified by real-time PCR following RNA extraction and reverse transcription (Fig 2A). Proliferation and cell viability were determined using the MTS assay and cell counting respectively (Fig 2B and C). For analysis of cell cycle, cells were fixed in 70% ethanol and stained with propidium iodide and subjected to flow cytometry as described in Materials and Methods (Fig 2D). For determination of apoptosis, cells were stained with Annexin V-FITC and Sytox Blue and analyzed by flow cytometry (Fig 2E).

Anita Balakrishnan, et al. Exp Cell Res. ;316(20):3512-3521.

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