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Results: 5

1.
FIG. 1.

FIG. 1. From: Development and Validation of an In Vivo Candida albicans Biofilm Denture Model .

Placement of rat denture. Stainless steel wire was threaded between cheek teeth. Acrylic denture material was applied over palate and around cheek teeth (8 by 10 mm). The spatula left a space (3 by 5 mm) for Candida inoculation.

Jeniel E. Nett, et al. Infect Immun. 2010 September;78(9):3650-3659.
2.
FIG. 5.

FIG. 5. From: Development and Validation of an In Vivo Candida albicans Biofilm Denture Model .

Histopathology of rat oral mucosa in the denture stomatitis model. Rat dentures were infected with C. albicans. After 48 h, animals were sacrificed, and dissected samples were fixed. Sections were stained with either hematoxylin and eosin (H&E) or Gomori's methenamine silver (GMS) for C. albicans. Images were obtained at 200× magnification.

Jeniel E. Nett, et al. Infect Immun. 2010 September;78(9):3650-3659.
3.
FIG. 4.

FIG. 4. From: Development and Validation of an In Vivo Candida albicans Biofilm Denture Model .

Confocal microscopy images of a C. albicans rat denture biofilm. (A) After 48 h of biofilm growth, rat dentures were harvested and stained with FUN-1 yeast viability stain (red-orange) and ConA (green) to visualize the fungal cells and matrix, respectively. The image was obtained at 63× magnification. (B) Rat dentures were imaged by confocal microscopy using calcofluor white (blue), SYTO 9 dye (green), and FUN-1 (red) to visualize fungal components and bacteria. Images were obtained at 100× magnification.

Jeniel E. Nett, et al. Infect Immun. 2010 September;78(9):3650-3659.
4.
FIG. 3.

FIG. 3. From: Development and Validation of an In Vivo Candida albicans Biofilm Denture Model .

Scanning electron microscopy (SEM) images of a C. albicans rat denture biofilm. (A) Rat dentures were harvested after 6, 24, or 48 h of growth, processed for SEM, and imaged. Scale bars for images at 50× and 1,000× magnification represent 600 μM and 30 μM, respectively. (B) Hyphal elements and bacteria are visualized in a denture biofilm imaged at 2,000× magnification.

Jeniel E. Nett, et al. Infect Immun. 2010 September;78(9):3650-3659.
5.
FIG. 2.

FIG. 2. From: Development and Validation of an In Vivo Candida albicans Biofilm Denture Model .

Microbiology of rat dentures infected with C. albicans biofilm infection. (A) Rat dentures were harvested after 6, 24, or 48 h of growth, and microbiological counting was used to determine the number of organisms present in the biofilms. (B) Rat dentures were harvested after 48 h of biofilm growth, and adherent bacteria were enumerated and categorized based on Gram staining, morphology, and aerobic growth. GN, Gram negative; GP, Gram positive. (C) Rats with dentures in place received no C. albicans inoculum (control), C. albicans inoculum only (infection), or treatment with ampicillin-sulbactam prior to C. albicans inoculum (infection + Amp-Sulb). Microbiological counting was used to determine the number of organisms present in the biofilms after 48 h of growth. All experiments were performed on two occasions, and microbiologic counting was performed in duplicate.

Jeniel E. Nett, et al. Infect Immun. 2010 September;78(9):3650-3659.

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