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Results: 5

1.
Image 4

Image 4. From: PCR-assays Detect B-lymphocyte Clonality in Formalin-fixed Paraffin Embedded Specimens of Classical Hodgkin Lymphoma without Microdissection.

Index Case 2: An excisional biopsy shows sheets of highly pleomorphic cells, with the same representative field shown at two magnifications (A and B). These cells are uniformly positive for CD30 (C).

W. Richard Burack, et al. Am J Clin Pathol. ;134(1):104-111.
2.
Image 1

Image 1. From: PCR-assays Detect B-lymphocyte Clonality in Formalin-fixed Paraffin Embedded Specimens of Classical Hodgkin Lymphoma without Microdissection.

Index Case 1: A core biopsy of a soft tissue mass shows a proliferation of lymphocytes and histiocytes intermixed with fibrosis (A). In a few regions, CD30+ mononuclear forms are abundant (B). A very rare form compatible with a Reed-Sternberg cell is seen (C).

W. Richard Burack, et al. Am J Clin Pathol. ;134(1):104-111.
3.
Image 3

Image 3. From: PCR-assays Detect B-lymphocyte Clonality in Formalin-fixed Paraffin Embedded Specimens of Classical Hodgkin Lymphoma without Microdissection.

PCR analysis of the IGK locus. The patient lanes (cases 1 and 2) were done in duplicate. The primer sets were designed to detect V-J joinings (BIOMED-2 IGK tube A), and IGK deletion (BIOMED-2 IGK tube B). The controls are the same as described in the legend for figure 3. The main clonal bands are indicated with arrowheads.

W. Richard Burack, et al. Am J Clin Pathol. ;134(1):104-111.
4.
Image 2

Image 2. From: PCR-assays Detect B-lymphocyte Clonality in Formalin-fixed Paraffin Embedded Specimens of Classical Hodgkin Lymphoma without Microdissection.

PCR analysis of the IGH locus. The patient lanes (cases 1 and 2) were done in duplicate. The upstream primers are from the FR2 or FR3 regions as indicated (BIOMED-2 IGH tubes B and C, respectively), and the downstream primer is from the J segment. The controls are polyclonal (normal lymphocyte), 10% + (10% monoclonal + 90% polyclonal), and HL60 (cell line DNA lacking IGH rearrangement). The result for case 1 shows polyclonal B-lymphocytes, and the result for case 2 shows no direct evidence for clonality, but very little signal.

W. Richard Burack, et al. Am J Clin Pathol. ;134(1):104-111.
5.
Image 5

Image 5. From: PCR-assays Detect B-lymphocyte Clonality in Formalin-fixed Paraffin Embedded Specimens of Classical Hodgkin Lymphoma without Microdissection.

Heteroduplex Analysis of the IGK locus. The IGK PCR analysis was done as described in Materials and Methods, except that for the lanes labeled N the PCR products were electrophoresed after PCR without the use of the heteroduplex procedure. The lanes labeled H were the same PCR products after heterduplexing. Three genomic DNA specimens were studied, normal polyclonal spleen (P), and two mixtures of DNA from clonal B-lymphocytes (10% and 5%) with polyclonal DNA. The figure shows that the heteroduplexing procedure spreads out the polyclonal signal and slows its migration so that it is easier to discern the clonal signal, even when diluted 20-fold with DNA from polyclonal lymphocytes.

W. Richard Burack, et al. Am J Clin Pathol. ;134(1):104-111.

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