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Results: 3

1.

Figure 1. From: Effects of Ionizing Radiation on Self Renewal and Pluripotency of Human Embryonic Stem Cells.

(A) Schematic of experimental setup. (B,C) Immunostaining of pluripotency markers in control (B) and irradiated (C) hESCs shows maintenance of marker expression 48 hours after irradiation. (D) Flow cytometry of FITC Annexin V and propidium iodide (PI) double-stained hESCs 48 hours after the indicated radiation dose (one representative experiment of three is shown).

Kitchener D. Wilson, et al. Cancer Res. ;70(13):5539-5548.
2.
Figure 3

Figure 3. From: Effects of Ionizing Radiation on Self Renewal and Pluripotency of Human Embryonic Stem Cells.

Microarray analysis of hESCs 24 hours after irradiation. (A) Pearson clustering of the data for 0, 0.4, 2, and 4 Gy irradiated hESCs (n=3 biological replicates per group). Note that one replicate from the 0.4 Gy group was lost due to poor array hybridization. Each gene is represented by a single row, and each sample by a single column. Red = upregulated, Green = downregulated. (B) Global Pearson correlation of microarray data. (C) Venn diagram of the significant entities (P<0.05, fold change ≥1.4) between each radiation group and control (0 Gy).

Kitchener D. Wilson, et al. Cancer Res. ;70(13):5539-5548.
3.
Figure 2

Figure 2. From: Effects of Ionizing Radiation on Self Renewal and Pluripotency of Human Embryonic Stem Cells.

Bioluminescence reporter gene imaging of irradiated hESCs in living animals. (A) The double fusion reporter gene construct carrying firefly luciferase (Fluc) and enhanced green fluorescent protein (eGFP). (B) Diagram of the subcutaneous injection sites for each radiation group plus control, as well as representative bioluminescent images for three mice through day 42. (C) Plot of longitudinal bioluminescent signal intensities for each group (*0 and 0.4 Gy vs. 2 and 4 Gy, P<0.05). Data presented as mean±SEM. (D) H&E staining of teratoma section from representative 4 Gy group demonstrating three embryonic germ layers.

Kitchener D. Wilson, et al. Cancer Res. ;70(13):5539-5548.

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