Results: 5

1.
Fig. 3

Fig. 3. From: Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF.

Illustration of single labeling methods for phi29 pRNA.

Hui Zhang, et al. Proc SPIE. ;7571:757107-757108.
2.
Fig. 1

Fig. 1. From: Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF.

Schematic drawing of the customized single-molecule imaging system which combines prism-type and objective-type TIR.

Hui Zhang, et al. Proc SPIE. ;7571:757107-757108.
3.
Fig. 2

Fig. 2. From: Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF.

Single-molecule study of procapsid/Cy3-pRNA complexes. (A) Fluorescence image of procapsid/Cy3-pRNA complexes. Each complex appeared as a single fluorescent spot. (B) Photobleaching trace of the procapsid/Cy3-pRNA complex.

Hui Zhang, et al. Proc SPIE. ;7571:757107-757108.
4.
Fig. 4

Fig. 4. From: Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF.

Cartoon showing the mechanism of the interaction between pRNA and procapsid. (A) The pRNA Aa’ can form a hexameric close ring and thus stays bound to procapsid. (B) The pRNA Ab’ cannot form a close ring, thus no stable procapsid/RNA complexes. (C) The pRNA Ab’ can be rescued by addition of the pRNA of Ba’ to form close ring and stays on procapsid.

Hui Zhang, et al. Proc SPIE. ;7571:757107-757108.
5.
Fig. 5

Fig. 5. From: Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF.

Single-molecule FRET studies of pRNA. The samples were excited with 532 nm laser and detections were achieved in both Cy3 and Cy5 channels using the Dual-View imager. After the combination of the two channels using the Field-Split function in Andor iQ software, each overlapped signal was analyzed to calculate the FRET efficiency. The data was then summarized to give a histogram in FRET efficiency, which was then fitted with Gaussian curve to obtain the mean efficiency.

Hui Zhang, et al. Proc SPIE. ;7571:757107-757108.

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