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Results: 5

1.
Fig. 2.

Fig. 2. From: Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone.

Undermineralization of bone in both Prkar1a+/− and Prkar1a+/−Prkaca+/− mice. (A) μCT images of caudal vertebra from WT, Prkaca+/−, Prkar1a+/− , and Prkar1a+/−Prkaca+/− mice at the age of 12 months. (B) Average of tissue mineral content (TMC) measurement of three caudal vertebrae from WT, Prkaca+/−, Prkar1a+/−, and Prkar1a+/−Prkaca+/− mice at 12 months old. **, P < 0.01. Error bars represent means ± SD.

Kit Man Tsang, et al. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8683-8688.
2.
Fig. 5.

Fig. 5. From: Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone.

Expression of mesenchymal proteins in bone lesions from Prkar1a+/− and Prkar1a+/−Prkaca+/− mice and epithelial markers in Prkar1a+/−Prkaca+/− bone tumors that confirms the mesenchymal-to-epithelial gene signature in lesions from Prkar1a+/−Prkaca+/− mice. (A) Immunohistochemistry for n-cadherin and vimentin, mesenchymal proteins, is increased in all animals of PKA defects. (B) Immunohistochemistry for e-cadherin and cytokeratin 18, epithelial proteins, is increased in double heterozygote animals only.

Kit Man Tsang, et al. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8683-8688.
3.
Fig. 4.

Fig. 4. From: Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone.

Increased PKA-II complex, type II regulatory subunit and catalytic subunit β1, and Prkx in Prkar1a+/−Prkaca+/− mice bone tumors. (A) DEAE-chromatography of PKA isozymes in tail tissues of WT and Prkaca+/− mice and tail lesions of Prkar1a+/− and Prkar1a+/−Prkaca+/− mice. PKA-II to PKA-I ratio was calculated from averaging the intensities of 10 fractions within the peaks. Note that tail lesions of Prkar1a+/−Prkaca+/− mice had the highest PKA-II to PKA-I ratio (n = 3). (B) Western blot analysis on RIIα, RIIβ, and phosphorylated form of RII in WT, Prkaca+/−, Prkar1a+/−, and Prkar1a+/−Prkaca+/− mice at 1 year of age, showing the up-regulation of RII subunits in bone lesions and increase in phosphorylated form of RII in Prkar1a+/−Prkaca+/− tumors. (C) Western blot analysis on different PKA catalytic subunits of WT, Prkaca+/−, Prkar1a+/−, and Prkar1a+/−Prkaca+/− mice at 1 year of age. (D) Relative quantification of Prkx, Cα, Cγ, Cβ1, and Cβ2 protein in bone lesions against WT normal bone.

Kit Man Tsang, et al. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8683-8688.
4.
Fig. 1.

Fig. 1. From: Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone.

Development of bone lesions along the tail of Prkar1a+/− and Prkar1a+/−Prkaca+/− mice. (A Left) comparison of tails from WT, Prkaca+/−, Prkar1a+/−, and Prkar1a+/−Prkaca+/− mice at 12 months old. (A Right) X-ray radiographs. White arrows point to the lesions. (B) Kaplan–Meier curve shows the number of tail masses found in various ages of Prkar1a+/− and Prkar1a+/−Prkaca+/− mice. (CG) Hematoxylin and eosin (H&E) staining of longitudinal sections of WT bones and Prkar1a+/−Prkaca+/− bone lesions. In C, black arrows denote the presence of mature osteoblasts lining along the trabecular bone. (Original magnification: C Upper, ×10; Lower, original magnification, ×20 . In D, the asterisk denotes the bone marrow space filled with fibroblastoid cells. (Original magnification, ×40). In E, black arrows denote the presence of osteoclasts. (Original magnification: ×20.) In F, the black arrow denotes the destroyed joint space. (Original magnification: ×2.) In G, the asterisk denotes the presence of cartilage island within the fibroblasts. (Original magnification: ×20.)

Kit Man Tsang, et al. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8683-8688.
5.
Fig. 3.

Fig. 3. From: Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone.

Structure and mineralization of cortical bone in adjacent affected and unaffected caudal vertebrae. (A) Brightfield and polarized images of unaffected and affected caudal vertebrae. Well organized, lamellar/fine-fibered bone was indicated by well oriented spindle-shaped osteocyte lacunae (arrows) and more uniform polarized images due to regular collagen fiber orientation. Woven bone was indicated by irregular-shaped, disoriented lacunae and patchy appearance of polarized images due to irregular fiber orientation. (B) Even (−0.1 ± 0.2 mm−1 slope) mineral/matrix ratio across the cortical layer (the intensity ratio of mineral PO4 to organic CH Raman peaks) is characteristic of well mineralized, mature bone in unaffected vertebrae. Gradually increasing mineral/matrix ratio from periosteal to endosteal surface [+0.8 ± 0.2 (SD) mm−1 slope, P < 0.003] indicates lagging mineralization characteristic of rapidly growing, immature bone in affected vertebrae. (C) High mineralization heterogeneity (coefficient of variation for the mineral/matrix ratio) in all cortical regions of affected vertebrae is also consistent with rapid formation of immature bone.

Kit Man Tsang, et al. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8683-8688.

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