Results: 5

1.
FIG. 4.

FIG. 4. From: Pertactin Is Required for Bordetella Species To Resist Neutrophil-Mediated Clearance .

Infection of SCID-beige mice. Groups of six mice were infected intranasally with phosphate-buffered saline (PBS) or 500 CFU of RB50 or SP5 and monitored for signs of disease. Shown are the percentages of surviving mice over time.

Carol S. Inatsuka, et al. Infect Immun. 2010 July;78(7):2901-2909.
2.
FIG. 5.

FIG. 5. From: Pertactin Is Required for Bordetella Species To Resist Neutrophil-Mediated Clearance .

Infections of neutropenic mice. Groups of six BALB/c mice rendered neutropenic by cyclophosphamide treatment were inoculated with PBS or 5 × 105 CFU of either RB50 or SP5. Shown are the percentages of surviving mice over time.

Carol S. Inatsuka, et al. Infect Immun. 2010 July;78(7):2901-2909.
3.
FIG. 2.

FIG. 2. From: Pertactin Is Required for Bordetella Species To Resist Neutrophil-Mediated Clearance .

Adherence of B. bronchiseptica strains to L2 (rat lung epithelial) and MH-S (murine alveolar macrophage-like) cell lines. Data from at least two independent experiments were averaged, and the standard deviations are shown (MOI = 50). Asterisks indicate a statistically significant difference compared with RB50.

Carol S. Inatsuka, et al. Infect Immun. 2010 July;78(7):2901-2909.
4.
FIG. 3.

FIG. 3. From: Pertactin Is Required for Bordetella Species To Resist Neutrophil-Mediated Clearance .

Colonization of mouse lungs. Shown are data for colonization of mouse lungs at 1 to 3 h (day 0), 3 days, and 11 days postinoculation with 5 × 105 CFU of wild-type or prn mutant strains. Each symbol indicates the number of CFU from a single animal. The horizontal bar indicates the geometric mean. For animals inoculated with the ΔfhaB strain, the means for the moribund animals (∼108 CFU) and healthy animals (∼106 CFU) were calculated separately. The dashed line represents the lower limit of detection. *, P < 0.05 compared with RB50.

Carol S. Inatsuka, et al. Infect Immun. 2010 July;78(7):2901-2909.
5.
FIG. 1.

FIG. 1. From: Pertactin Is Required for Bordetella Species To Resist Neutrophil-Mediated Clearance .

Construction of prn mutants. (A) SP5, the Δprn mutant, was created using allelic exchange to delete codons 227 to 756 of prn from the RB50 chromosome. The Δprn mutation was complemented with plasmid pVC4, a suicide plasmid containing the wild-type (wt) prn gene and its entire promoter region. The integration of plasmid pVC4 in the region 5′ of the deletion in SP5 (as shown) leaves the region 3′ of the deletion in SP5 unaltered. RBXQ7 and RB545 were constructed by allelic exchange using pSS4245 as described in the text. (B) pSS4245 is an allelic exchange plasmid for use in Bordetella species. The positions of antibiotic resistance genes, the I-SceI restriction endonuclease-encoding gene (I-SceI CDS), the I-SceI recognition site, the ptx promoter, transcription terminators (TrrnB), and the origin of replication (oriV) are shown. (C) Whole-cell lysates of wild-type and prn mutants producing PRN proteins with HA epitopes at their N termini were probed with anti-HA antibody. The position of the 75-kDa molecular mass marker is shown.

Carol S. Inatsuka, et al. Infect Immun. 2010 July;78(7):2901-2909.

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