Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 11

1.
FIGURE 2.

FIGURE 2. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Scatter plot of normalized IP and WCE signal intensities of POL2, H3K4me2, H3ac, and H3K27me3 in U251 and MCF-7 cells. The solid line represents the median IP/WCE distribution of the respective mark across the array. Fractions enriched in the respective mark are in red.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
2.
FIGURE 11.

FIGURE 11. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

MiRNA profiling in U251 and MCF-7 cells. The results are shown as a Venn diagram. U251 and MCF-7 cells are in blue and red, respectively. Numbers are the individual miRNA species that were expressed only by MCF-7 cells (red circle), by U251 cells (blue circle), and by both cell types (overlap). The identity of the individual miRNAs is shown in the respective panels. Only miRNA expressed in either or both cell lines and p < 0.05 are shown. The results of the profiling are summarized in supplemental Table S5 and Fig. S3.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
3.
FIGURE 6.

FIGURE 6. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Epigenetic control of MMP-7, MMP-20, MMP-27, MMP-8, MMP-10, MMP-1, MMP-3, MMP-12, and MMP-13 from the 430-kb-long 11q22.3 region. The solid line represents the median IP/WCE ratio of the respective mark across the array. Enrichment of the mark is above the line. The data are presented in a log2 format. The gene promoters are schematically shown at the bottom of the panels. U251 and MCF-7 cells are in blue and red, respectively. The arrows show the position of the respective gene in the cluster and the direction of transcription, respectively.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
4.
FIGURE 1.

FIGURE 1. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Gene expression profiling of U251 cells and MCF-7 cells. Only the invasion-related genes included in the custom 486-gene ChIP-on-ChIP microarray are shown. A, horizontal axis, signal intensity of the individual genes (the log scale) is shown. The 56 and 14 genes up-regulated in U251 and MCF-7 cells, respectively, are shown. B, RT-PCR analysis of the selected genes is shown: matrix metalloproteinases (MMP-2, MMP-7, MT1-MMP, MT2-MMP, MT3-MMP, MT4-MMP and MT6-MMP), TIMP-4, MIF, and collagens (COL11A1, COL7A1, COL8A1, COL5A1 and COL18A1). GAPDH was used as a loading control.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
5.
FIGURE 8.

FIGURE 8. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Epigenetic signatures of MMP-13, MT2-MMP, MT3-MMP, MT4-MMP, MMP-20, MT5-MMP, MT6-MMP, MMP-27, and furin. The solid line represents the median IP/WCE ratio of the respective mark across the array. Enrichment of the mark is above the line. The data are presented in a log2 format. The positions of the respective probes are mapped relative to the transcription initiation start of the gene promoter (shown at the bottom of the panels). U251 and MCF-7 cells are in blue and red, respectively. Black bars correspond to the first exon. The arrows show the direction of transcription. Gray bars show the CpGi regions. Thin solid bars correspond to the 1 kb-size genomic fragments.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
6.
FIGURE 7.

FIGURE 7. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Epigenetic signature of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, and MMP-12. The solid line represents the median IP/WCE ratio of the respective mark across the array. Enrichment of the mark is above the line. The data are presented in a log2 format. The positions of the respective probes are mapped relative to the transcription initiation start of the gene promoter (shown at the bottom of the panels). U251 and MCF-7 cells are in blue and red, respectively. Black bars correspond to the first exon. The arrows show the direction of transcription. Gray bars show the CpGi regions. Thin solid bars correspond to the 1-kb genomic fragments.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
7.
FIGURE 10.

FIGURE 10. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Expression of collagen genes in glioma samples. A, the quantitative-PCR analysis was used to measure the levels of mRNAs coding for selected collagens (COL11A1, COL7A1, COL8A1, COL5A1v and COL1A1) in U251 cells, U251 non-orthotopic xenograft in mice, and several GBM grade IV specimens. For simplicity, only the patient sample 2394 is shown in the panel. GAPDH was used as a control. Each sample was analyzed in triplicate. Relative mean cycle threshold (ΔCt) values are shown. B, the microarray data was derived from the ONCOMINE databases, Sun brain data subset. The subset includes 23 human normal brain samples and 81 GBM specimens. Red and blue colors denote high and low levels of expression.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
8.
FIGURE 5.

FIGURE 5. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Epigenetic control of TIMPs in U251 and MCF-7 cells. A, the TIMP-1, -2, -3, and -4 mRNA levels (±S.E.) in the cells are shown. The data are derived from the gene expression analysis. The cut-off level (50 intensity units) is shown as a dotted line. B, shown is the epigenetic signature of TIMPs in U251 and MCF-7 cells. The solid line represents the median IP/WCE ratio of the respective mark across the array. Enrichment of the mark is above the line. The data are presented in a log2 format. The positions of the respective probes are mapped relative to the transcription initiation start of the gene promoter (shown at the bottom of the panels). U251 and MCF-7 cells are in blue and red, respectively. Black bars correspond to the first exon. The arrows show the direction of transcription. Gray bars show the CpGi regions. Thin solid bars correspond to the 1-kb-size genomic fragments.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
9.
FIGURE 3.

FIGURE 3. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Epigenetic signature of the selected, transcriptionally active, pro-invasive genes in U251 cells. Chemokine (C-C motif) ligand 2 (CCL2), connective tissue growth factor (CTGF), tenascin C (TNC), osteonectin (SPARC), cadherin 2 (CDH2), laminin β1 (LAMB1), cadherin 6 (CDH6), versican (VCAN), secreted phosphoprotein 1 (SPP1), and integrin α4 (ITGA4) are shown. The solid line represents the median IP/WCE ratio of the respective mark across the array. Enrichment of the mark is above the line. The data are presented in a log2 format. The positions of the respective probes are mapped relative to the transcription initiation start of the gene promoter (shown at the bottom of the panels). U251 and MCF-7 cells are in blue and red, respectively. Black bars correspond to the first exon. The arrows show the direction of transcription. Gray bars show the CpGi regions. Thin solid bars correspond to the 1-kb-size genomic fragments.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
10.
FIGURE 4.

FIGURE 4. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Epigenetic signature of the selected, transcriptionally active pro-invasive genes in MCF-7 cells. Macrophage migration inhibitory factor (MIF); chemokine (CXC motif) ligand 12 (CXCL12), bone morphogenetic protein 4 (BMP4), cadherin 1 (CDH1), par-6 partitioning defective 6 homolog beta (PARD6B), chemokine (CXC motif) receptor 4 (CXCR4), metastasis suppressor 1 (MTSS1), spleen tyrosine kinase (SYK), achaete-scute complex homolog 2 (ASCL2), and hairy/enhancer-of-split related protein (HEYL) are shown. The solid line represents the median IP/WCE ratio of the respective mark across the array. Enrichment of the mark is above the line. The data are presented in a log2 format. The positions of the respective probes are mapped relative to the transcription initiation start of the gene promoter (shown at the bottom of the panels). U251 and MCF-7 cells are in blue and red, respectively. Black bars correspond to the first exon. The arrows show the direction of transcription. Gray bars show the CpGi regions. Thin solid bars correspond to the 1-kb-size genomic fragments.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.
11.
FIGURE 9.

FIGURE 9. From: Microarray-based Transcriptional and Epigenetic Profiling of Matrix Metalloproteinases, Collagens, and Related Genes in Cancer.

Epigenetic signature of collagens. A, the mRNA levels (±S.E.) of the 18 individual collagens in the cells are shown. The data are derived from the gene expression analysis. B, shown is the epigenetic signature of the selected collagens in U251 and MCF-7 cells. COL11A1, COL6A1, COL7A1, COL4A1, COL4A2, COL5A1, COL18A1, COL8A1, COL12A1, COL1A1, and COL9A1 are shown. The solid line represents the median IP/WCE ratio of the respective mark across the array. Enrichment of the mark is above the line. The data are presented in a log2 format. The positions of the respective probes are mapped relative to the transcription initiation start of the gene promoter (shown at the bottom of the panels). Black bars correspond to the first exon. The arrows show the direction of transcription. Gray bars show the CpGi regions. Thin solid bars correspond to the 1-kb genomic fragments. U251 and MCF-7 cells are in blue and red, respectively. C, collagen immunopositivity of human U251 glioma tumor xenograft in the mouse brain is shown. Left, the orthotopic U251 xenografts in the mouse brain were stained with hematoxylin and eosin. The inset shows the enlarged tumor area. Middle, the orthotopic U251 xenografts in the mouse brain were stained with the antibodies to human nucleobindin 1 (a human tumor cell marker; green) and type I collagen (red). The nuclei were stained with 4,6-diamidino-2-phenylindole (blue). Note the collagen (red), produced by the glioma loci (green). Magnification, ×100. Right, collagen (red; arrow), produced by the murine brain neural cells, which are adjacent to glioma loci. Note that murine cells show no human nucleobindin 1 immunoreactivity. Nucleobindin 1-positive human glioma cells (green) are clearly visible in the upper right portion of the image. Magnification, ×400.

Andrei V. Chernov, et al. J Biol Chem. 2010 June 18;285(25):19647-19659.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk