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Results: 3

1.
Figure 2

Figure 2. From: Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters.

A) Profiling of E. coli lipoproteins with alk-FA reporters. B) Specific labeling of NlpE-His6 with alk-14. Coomassie blue staining (CB) indicates level of protein loading.

Kavita J. Rangan, et al. J Am Chem Soc. ;132(31):10628-10629.
2.
Figure 1

Figure 1. From: Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters.

A) Structure of a bacterial lipoprotein. (n = 12 – 20). B) Two-step bioorthogonal labeling strategy using alkynyl-fatty acids.

Kavita J. Rangan, et al. J Am Chem Soc. ;132(31):10628-10629.
3.
Figure 3

Figure 3. From: Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters.

Validation of alk-FA labeled proteins in bacteria. A) GO annotation of high confidence candidates identified from E. coli. Percent LPPs in each category is indicated in parenthesis. B. alk-14 labeling of SlyB and YggG. C) alk-14 labeling of YjgF and YjgFC107S. D) Competition of alk-14 labeling of YjgF with palmitic acid (PA) and sodium acetate (SA).

Kavita J. Rangan, et al. J Am Chem Soc. ;132(31):10628-10629.

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