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Results: 5

1.
Figure 5

Figure 5. From: DNA damage intensity in fibroblasts in a 3-dimensional collagen matrix correlates with the Bragg curve energy distribution of a high LET particle.

3D reconstructions of deconvolved images from nuclei with streaked (panel A; sample sectioned parallel to beam path, white arrow illustrating direction of particle path) and nuclear saturation damage (panel B; sample sectioned transverse to the particle path, particle enters cell head-on). Left-most unprocessed epifluorescent images obtained at 40× objective magnification while right-sided unprocessed images were obtained at 60× objective magnification. 3-D reconstructions (right-most images, top and side views of the nuclei) demonstrate a surface rendition of the γ-H2AX and DNA-PKcs damage.

Andres I. Roig, et al. Int J Radiat Biol. ;86(3):194-204.
2.
Figure 1

Figure 1. From: DNA damage intensity in fibroblasts in a 3-dimensional collagen matrix correlates with the Bragg curve energy distribution of a high LET particle.

(A) Collagen-fibroblast plugs are made from lung fibroblast cells grown in conventional monolayer. Once mixed with collagen to make a three dimensional tissue equivalent, they are properly oriented for exposure to high LET radiation at the NASA Space Radiation Laboratory (NSRL) at Brookhaven National Laboratory (BNL). (B) Placement of plugs at different positions in the Bragg curve of a 307.7 MeV/nucleon 56Fe particle shows varying energies deposited to tissues depending on their location in the Bragg curve.

Andres I. Roig, et al. Int J Radiat Biol. ;86(3):194-204.
3.
Figure 4

Figure 4. From: DNA damage intensity in fibroblasts in a 3-dimensional collagen matrix correlates with the Bragg curve energy distribution of a high LET particle.

Magnified pictures of representative cell nuclei from specimens with saturated nuclear damage or mixed streaked- saturated nuclear damage for a 307.7 MeV/nucleon 56Fe beam. Both images come from samples sectioned parallel to the beam path. (A). Close up view of the saturated nuclear damage pattern processed with an epifluorescent microscope and corresponding deconvolved image sequences of the saturated damage revealing distinct particle tracks at multiple optical sections. Unprocessed image obtained at 40× objective magnification. Deconvolved images obtained at 60× objective magnification. (B). Sample nucleus with mixed streaked-saturated damage. Deconvolution shows a particle track with small tangential secondary tracks. Unprocessed image obtained at 60× magnification. Deconvolved images obtained at 60× objective magnification.

Andres I. Roig, et al. Int J Radiat Biol. ;86(3):194-204.
4.
Figure 3

Figure 3. From: DNA damage intensity in fibroblasts in a 3-dimensional collagen matrix correlates with the Bragg curve energy distribution of a high LET particle.

(A), (B), (C) Normalised DNA damage intensities calculated by averaging the mean grey scale values of the DNA damage intensities corresponding to 56Fe ions with 307.7 MeV/nucleon, 556.9 MeV/nucleon, or 967.0 MeV/nucleon energy super-imposed over the physical Bragg profiles for each particle energy. Four separate intensity measuring experiments (between 80 and 100 total cell nuclei) were performed for each spatial location in the Bragg curve for each particular energy as well for non-irradiated controls. Error bars indicate the standard error for the mean (SEM) for these four separate intensity measuring experiments.

Andres I. Roig, et al. Int J Radiat Biol. ;86(3):194-204.
5.
Figure 2

Figure 2. From: DNA damage intensity in fibroblasts in a 3-dimensional collagen matrix correlates with the Bragg curve energy distribution of a high LET particle.

(A), (B), (C) DNA damage in nuclei of specimens placed at various locations along the Bragg peak for 307.7 MeV/nucleon, 556.9 MeV/nucleon, or 967.0 MeV/nucleon 56Fe ions, respectively. The beam direction for each energy is indicated next to the left of each panel. The damage is appreciated as either discrete damage streaks or foci (white arrows) or saturated nuclear damage. All images taken at 40× objective magnification. (D) Percentage of nuclei in a 307.7 MeV/nucleon specimen showing streaks/foci or nuclear saturation damage. Co-localised γ-H2AX and DNA-PKcs nuclear streak damage is more prevalent in distances farther away from the Bragg peak while saturated nuclear damage is more common closer to the peak. Error bars indicate the standard error for the mean (SEM) for three separate counting experiments.

Andres I. Roig, et al. Int J Radiat Biol. ;86(3):194-204.

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