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1.
Fig 1

Fig 1. From: Antigen Engineering Can Play A Critical Role in the Protective Immunity Elicited by Yersinia pestis DNA Vaccines.

Comparison of codon usage frequency (%) for each amino acid between the wild type and codon optimized lcrV genes.

Shixia Wang, et al. Vaccine. ;28(8):2011-2019.
2.
Fig 4

Fig 4. From: Antigen Engineering Can Play A Critical Role in the Protective Immunity Elicited by Yersinia pestis DNA Vaccines.

A. Schematic diagram of gene inserts in F1 DNA vaccine constructs. The numbers indicate the amino acid positions. The weaved or solid boxes represent the putative F1 natural leader or tPA-leader sequence, respectively. (b) Western-blot analysis of the F1 protein expression by different F1 DNA vaccines in supernatant (S) or lysate (L) of transfected 293T cells.

Shixia Wang, et al. Vaccine. ;28(8):2011-2019.
3.
Fig 5

Fig 5. From: Antigen Engineering Can Play A Critical Role in the Protective Immunity Elicited by Yersinia pestis DNA Vaccines.

A. F1-specific IgG responses induced by different F1 DNA vaccines in Balb/C mice. The F1-specific IgG titers were measured by ELISA. Results shown were mean titers of each group (5 mice per group) after the 4th DNA immunization. B. Protection of mice immunized with different F1 DNA vaccines. The Balb/C mice were challenged with a lethal dose of 5000 cfu Y. pestis by intranasal inoculation at one week after the 4th DNA immunization. Cumulative survival curves were plotted to show the protection for each group as indicated. Vector control group was included as the negative control.

Shixia Wang, et al. Vaccine. ;28(8):2011-2019.
4.
Fig 2

Fig 2. From: Antigen Engineering Can Play A Critical Role in the Protective Immunity Elicited by Yersinia pestis DNA Vaccines.

Analyses V protein expression by the wild type (wt.V) or codon optimized (V.opt) LcrV DNA vaccine. A. Western-blot analysis of V protein expression by V.opt or wt.V DNA vaccines, or empty vector in supernatant (S) and lysate (L) of transfected 293T cells. B. V protein amount was determined by a quantitative ELISA in 293T cell lysate transfected with either V.opt or wt.V DNA vaccines. The V protein concentration in 293T cell lysate was calculated based on a standard curve of a control recombinant V protein. V-specific mouse sera were used as the detection antibody for Western blot (1:500 dilution) or ELISA (1:1000 dilution).

Shixia Wang, et al. Vaccine. ;28(8):2011-2019.
5.
Fig 6

Fig 6. From: Antigen Engineering Can Play A Critical Role in the Protective Immunity Elicited by Yersinia pestis DNA Vaccines.

A. Schematic diagram of caf1 and yscF fusion gene inserts in DNA vaccines. The numbers indicate the amino acid positions. The black, open or grey boxes represent the tPA-leader sequence, YscF and dF1 genes, respectively. B. Western-blot analysis of the YscF-dF1 or dF1-YscF fusion proteins using supernatant (S) and lysate (L) from 293T cells transiently transfected with different DNA vaccines or empty vector. dF1 DNA vaccine and vector was used as positive and negative controls. F1-specific mouse sera were used as detection antibody at 1:500 dilution.

Shixia Wang, et al. Vaccine. ;28(8):2011-2019.
6.
Fig 7

Fig 7. From: Antigen Engineering Can Play A Critical Role in the Protective Immunity Elicited by Yersinia pestis DNA Vaccines.

Antigen-specific responses and in vivo protection induced by YscF-dF1 and dF1-YscF DNA vaccine in Balb/C mice. YscF-2 and tPA-F1 DNA vaccines or empty vector were used as positive or negative controls, respectively. A. F1-specific IgG titers in immunized mouse sera. B. YscF-specific IgG titers in mouse immunized sera. The F1- or YscF-specific IgG titers were measured by ELISA using F1 or YscF-2 as coating antigen, respectively. Results shown here were mean titers of each group (5 mice per group) after the 4th DNA immunization. B. In vivo protections of the Balb/C mice against intranasal challenge with a lethal dose of 5000 cfu Y. pestis at one week after the 4th DNA immunization. Cumulative survival curves were plotted to show the protection for each group as indicated.

Shixia Wang, et al. Vaccine. ;28(8):2011-2019.
7.
Fig 3

Fig 3. From: Antigen Engineering Can Play A Critical Role in the Protective Immunity Elicited by Yersinia pestis DNA Vaccines.

V-specific immune protection induced by V DNA vaccines. A. Temporal V-specific antibody responses of group-pooled sera at 1:500 serum dilution. The arrows indicate the time-points of DNA immunizations. B. Peak levels of V-specific antibody titers after the 4th DNA immunization. The data show mean titers with standard deviations of 10 Balb/C mice in each group. Statistical difference is indicated between V.opt and wt.V DNA immunization groups. C. The IgG1/IgG2a ratios in V.opt and wt.V DNA immunized mice at peak antibody responses. D. In vivo protections of mice immunized with different V DNA vaccines as indicated. The Balb/C mice were challenged with a lethal dose of 80000 cfu Y. pestis by intranasal inoculation at one week after the 4th DNA immunization. The percent of survival of each group of 10 Balb/C mice is shown. * indicates the significant difference (p < 0.05) of survival rates between V.opt and wt.V DNA immunization groups.

Shixia Wang, et al. Vaccine. ;28(8):2011-2019.

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