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2.
Figure 4

Figure 4. From: Enhanced Tumor Cell Isolation by a Biomimetic Combination of E-selectin and anti-EpCAM: Implication for Effective Separation of Circulating Tumor Cells (CTCs).

Cell rolling velocities of HL-60 and MCF-7 cells on E-selectin-immobilized slides at various shear stresses (0.08 dyn/cm2, 0.32 dyn/cm2, 0.64 dyn/cm2, and 1.28 dyn/cm2). Note that the rolling response of HL-60 cells is minimally affected by an increase in shear stress, whereas MCF-7 cells show rolling highly dependent upon shear stress. Error bars: standard error.

Ja Hye Myung, et al. Langmuir. ;26(11):8589-8596.
3.
Figure 5

Figure 5. From: Enhanced Tumor Cell Isolation by a Biomimetic Combination of E-selectin and anti-EpCAM: Implication for Effective Separation of Circulating Tumor Cells (CTCs).

Images of HL-60 and DsRED-transfected MCF-7 cells (red cells) on (a) P-selectin, (b) E-selectin, (c) anti-EpCAM, and (d) patterned E-selectin/anti-EpCAM coated surfaces, under shear stress of 0.32 dyn/cm2. The patterned surface with E-selectin and anti-EpCAM shown in (d) achieved efficient isolation of DsRED-transfected MCF-7 (a CTC model: red cells) cells from the mixture with HL-60 (a leukocyte model: white cells), on the anti-EpCAM coated region.

Ja Hye Myung, et al. Langmuir. ;26(11):8589-8596.
4.
Figure 2

Figure 2. From: Enhanced Tumor Cell Isolation by a Biomimetic Combination of E-selectin and anti-EpCAM: Implication for Effective Separation of Circulating Tumor Cells (CTCs).

Time-course images of HL-60 cells under shear stress of 0.32 dyn/cm2 on (a) and (b) P-selectin, (c) and (d) E-selectin, and (e) and (f) anti-EpCAM-immobilized surfaces. The rolling velocities (mean ± standard error, n=200) of the cells on P-selectin and E-selectin were 2.26 ± 0.28 and 2.12 ± 0.15 μm/sec, respectively, whereas there was no interaction observed between the cells and anti-EpCAM-coated surface. The cells in the images (e) and (f) are non-interacting flowing cells. Flow direction of the three sets is from left to right.

Ja Hye Myung, et al. Langmuir. ;26(11):8589-8596.
5.
Figure 7

Figure 7. From: Enhanced Tumor Cell Isolation by a Biomimetic Combination of E-selectin and anti-EpCAM: Implication for Effective Separation of Circulating Tumor Cells (CTCs).

Effect of the amount of E-selectin added to E-selectin/anti-EpCAM mixture on rolling velocity of HL-60 cells and capture efficiency of DsRED-MCF-7 cells. Mixture of the two cell populations (1:1) were injected onto the surfaces co-immobilized with anti-EpCAM and E-selectin under the presence of anti-IgG at a shear stress of 0.16 dyn/cm2. The amount of immobilized E-selectin was increased from 0, 0.3, and 1.5 to 7.5 μg, while the amount of immobilized anti-EpCAM was constant at 1.5 μg. The rolling velocities of HL-60 cells on each slides were 4.74 ± 0.32 (0.3 μg), 1.82 ± 0.10 (1.5 μg), and 0.07 ± 0.12 (7.5 μg of E-selectin) μm/sec. Error bars: standard error.

Ja Hye Myung, et al. Langmuir. ;26(11):8589-8596.
6.
Figure 3

Figure 3. From: Enhanced Tumor Cell Isolation by a Biomimetic Combination of E-selectin and anti-EpCAM: Implication for Effective Separation of Circulating Tumor Cells (CTCs).

Time-course images of MCF-7 cells under shear stress of 0.32 dyn/cm2 on (a) and (b) P-selectin, (c) and (d) E-selectin, and (e) and (f) anti-EpCAM-immobilized surfaces. MCF-7 cells exhibited the rolling behavior on the E-selectin-coated surface (4.24 ± 0.31 m/sec) or captured on the anti-EpCAM-coated surface. However, there was no interaction observed between the cells and the P-selectin-coated surface. Flow direction of the three sets is from left to right. All of the rolling dynamic data is represented as mean ± standard error (n=200).

Ja Hye Myung, et al. Langmuir. ;26(11):8589-8596.
7.
Figure 6

Figure 6. From: Enhanced Tumor Cell Isolation by a Biomimetic Combination of E-selectin and anti-EpCAM: Implication for Effective Separation of Circulating Tumor Cells (CTCs).

(a) Number of captured cells and (b) capture efficiencies of the surfaces immobilized with the mixtures of anti-EpCAM and E-selectin. The number of DsRED-MCF-7 cells on each surface was counted and the capture efficiency was calculated based on the total number of MCF-7 cells injected into the flow chamber. The flow experiments were performed at a shear stress of 0.16 dyn/cm2. The average capture efficiencies of the surfaces with the mixture of E-selectin and anti-EpCAM were generally higher than those with anti-EpCAM alone. With an increase in E-selectin concentration, the capture efficiency of the surfaces was further enhanced as high as 3-fold. The measured capture efficiencies were compared by statistical analysis using one-factor ANOVA, followed by Fisher’s least significant difference (LSD) tests with 95% simultaneous confidence intervals (SPSS software). Error bars: standard error. * p < 0.05.

Ja Hye Myung, et al. Langmuir. ;26(11):8589-8596.

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