Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 6

1.
Figure 1

Figure 1. From: Nanotopographical modification: a regulator of cellular function through focal adhesions.

The central dogma of tissue engineering. Cells are isolated and combined in vitro with a suitable scaffold system. Culture systems are used to encourage cellular infiltration and proliferation before being transplanted to a site of disease or compromise.

Manus Jonathan Paul Biggs, et al. Nanomedicine. ;6(5):619-633.
2.
Figure 3

Figure 3. From: Nanotopographical modification: a regulator of cellular function through focal adhesions.

A simplified overview of the molecular interactions occurring at the focal adhesion. Focal adhesions are macromolecular structures that serve as mechanical linkages of the cell cytoskeleton (F-actin) to the extracellular matrix (ECM), and as biochemical signaling hubs involved with the transmission of external mechanical forces to changes in cell function through the regulated interactions of focal adhesion associated signaling molecules.

Manus Jonathan Paul Biggs, et al. Nanomedicine. ;6(5):619-633.
3.
Figure 5

Figure 5. From: Nanotopographical modification: a regulator of cellular function through focal adhesions.

The influence of nanoscale protrusions on focal adhesion formation and reinforcement. (A) Integrin clustering and focal adhesion reinforcement is unaffected on nanoscale protrusions with a critical spacing of <70 nm and a nanoprotrusion diameter of >70 nm. (B) Increasing the interfeature spacing to the submicron scale facilitates cell-basal substratum interactions below a feature height of <70 nm. (C) Conversely, increasing the feature height restricts integrin binding to the planar basal substrate and restricts focal adhesion formation to the feature apexes. (D) Integrin clustering and cellular adhesion is greatly perturbed on nanoscale protrusion with a feature diameter of <70 nm and an interfeature distance >70 nm.

Manus Jonathan Paul Biggs, et al. Nanomedicine. ;6(5):619-633.
4.
Figure 2

Figure 2. From: Nanotopographical modification: a regulator of cellular function through focal adhesions.

Cell-substrate interactions and focal adhesion formation. (A) Adherent cells form dynamic actin-rich extensions during the process of cellular spreading and migration and (B) probe the underlying (grooved) substratum with fine filopodial extensions (arrows) from the leading and trailing free edge. (C) Adherent cells maintain cellular integrity through a dynamic network of contractile actin stress fibers (red) that terminate in focal adhesion plaques (green), molecular complexes that intimately connect the cytoskeleton with the extracellular matrix.

Manus Jonathan Paul Biggs, et al. Nanomedicine. ;6(5):619-633.
5.
Figure 6

Figure 6. From: Nanotopographical modification: a regulator of cellular function through focal adhesions.

The influence of nanoscale pits on focal adhesion formation and reinforcement. (A) Integrin clustering and focal adhesion reinforcement is unaffected on nanoscale pits with a diameter of <70 nm irrespective of pit depth. (B) Increasing the pit diameter to >70 nm perturbs integrin clustering when the z dimensions of the pits exceed <100 nm. (C) Conversely, increasing the pit x-y dimensions and reducing the z dimensions facilitates integrin clustering and focal adhesion formation on the basal planar surface and at the base of the pits. (D) Integrin clustering and cellular adhesion is greatly perturbed on nanoscale pits with a feature diameter between 70 and 300 nm and an interpit separation of <70 nm.

Manus Jonathan Paul Biggs, et al. Nanomedicine. ;6(5):619-633.
6.
Figure 4

Figure 4. From: Nanotopographical modification: a regulator of cellular function through focal adhesions.

Nanoscale topographical features influence cellular spreading and focal adhesion formation. (A) Nanoprotrusion with microscale x-y dimensions and a z dimension >70 nm increases cellular spreading. Nanoisland topography increases cellular spreading by providing tactile stimuli. (B) Immuno-gold labeling of focal adhesions (electron-dense clusters) in adherent cells allows the visualization of cell-substratum interactions. Nanoscale pits >70 nm in diameter perturb integrin clustering forcing adhesion formation to occur at the interpit regions. (C) Focal adhesions as visualized by scanning electron microscopy and immuno-gold labeling indicate that grooves with z dimensions down to a minimum 30–40 nm can induce adhesion alignment to the groove orientation.

Manus Jonathan Paul Biggs, et al. Nanomedicine. ;6(5):619-633.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk