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Results: 6

1.
Fig. 6

Fig. 6. From: Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient.

Npm expression by tumor and normal cells. Lysates of tumor cell lines and normal tissues were tested for Npm levels with anti-Npm antibody by Western blot analysis.

Rolf K. Swoboda, et al. Int J Cancer. ;127(5):1124-1130.
2.
Fig. 3

Fig. 3. From: Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient.

Cytotoxic activity of peptide 2-stimulated PBMC of HLA-A1 positive CRC patients and healthy donors against different target cells. PBMC were stimulated twice with peptide 2-pulsed monocytes in vitro and tested for cytotoxic activity against different target cells in 51Cr release assay.

Rolf K. Swoboda, et al. Int J Cancer. ;127(5):1124-1130.
3.
Fig. 2

Fig. 2. From: Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient.

Recognition of peptide-pulsed WM793 cells or 007 monocytes by CTL007
A. HLA-A1-positive WM793 cells were pulsed with the different Npm or control peptides and incubated with CTL007. CTL activity of CTL007 was measured in standard 51Cr release assay. B. HLA-A1 positive 007 monocytes were pulsed with different concentrations of peptide 2 or control peptide and the proliferative response of CTL007 was measured. The proliferative response to stimulation with peptide 2-pulsed monocytes was statistically significant (p<0.01).

Rolf K. Swoboda, et al. Int J Cancer. ;127(5):1124-1130.
4.
Fig. 5

Fig. 5. From: Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient.

Phenotype of CRC patients' PBMC stimulated with peptide 2. PBMC were sorted into CD4+ and CD8+ cells using magnetic beads. The two subpopulations as well as the unsorted PBMC were tested for cytotoxic activity in a CTL assay using peptide 2-loaded (solid line) and control-peptide loaded (dashed lines) WM793 cells or WC007 cells as target cells.

Rolf K. Swoboda, et al. Int J Cancer. ;127(5):1124-1130.
5.
Fig. 1

Fig. 1. From: Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient.

A. Stimulation of CTL007 by cDNA clone 2H3. HEK293 cells were treated as indicated and CTL007 added after 24 hours. IFN-γ release by HEK293 cells transfected with both HLA-A1 and 2H3 cDNA is statistically significantly higher (p<0.01) than the release by each of the control cells. Bars indicate mean IFN-γ release +/− S.D. of triplicate determinations. B. WC007 cells were stably transduced with Npm-specific shRNA lentiviral particles (#68–72) or mock transduced. Lysis of the target cells was determined in live/dead viability/cytotoxicity assay.

Rolf K. Swoboda, et al. Int J Cancer. ;127(5):1124-1130.
6.
Fig. 4

Fig. 4. From: Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient.

A. Phenotype of 007PBMC stimulated with peptide 2. PBMC of patient 007 were stimulated twice with peptide 2 and after 3 weeks the cells were analyzed for binding to anti-CD4 (dashed line), anti-CD8 (dotted line) or isotype - matched control (solid line) antibodies by FACS analysis. B. PBMC were sorted into CD4+ and CD8+ cells using magnetic beads. The two subpopulations as well as the unsorted PBMC were used in a CTL assay using WC007 as target cells

Rolf K. Swoboda, et al. Int J Cancer. ;127(5):1124-1130.

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