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1.
Figure 6

Figure 6. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Effect of δ-opioid receptor (δOR) antagonist pretreatment on the sensitization to morphine conditioned place preference (CPP). Animals were pretreated with naltriben (0.1 mg/kg, s.c.) 15 min before the administration of morphine both during sensitization and conditioning as described in ‘Materials and methods' section. Data represent the change of preference as described above (mean±SEM). One-way ANOVA with Tukey's test for multiple comparisons, n=6–8, ***p<0.001.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.
2.
Figure 2

Figure 2. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Modulation of mitogen-activated protein kinase (MAPK), calcium/calmodulin-dependent kinase II (CaMKII), GluR1, and CREB 48 h after acute morphine administration. Rats treated with a single injection of saline or morphine (10 mg/kg, s.c.) were killed 48 h later and the brain regions were subjected to western blotting analysis using antibodies against pMAPK, total MAPK (a), pCaMKII, total CaMKII (b), pGluR1, total GluR1 (c), pCREB, and actin (d) as described in ‘Materials and methods' section. A representative western blot is shown for each of the proteins studied. Data from multiple experiments (4–6 animals per group) were tabulated and presented as relative ratio of phosphorylated/total forms (mean±SEM). One-way ANOVA with unpaired two-tailed t-test, n=4–6, *p<0.05.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.
3.
Figure 7

Figure 7. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Effect of δ-opioid receptor (δOR) antagonist pretreatment on the induction of pCREB levels. The paradigm used for development of sensitization to morphine was modified in that animals were pretreated with naltriben (0.1 mg/kg, s.c.) 15 min before the administration of morphine both during sensitization and conditioning as described in ‘Materials and methods' section. Brain regions were dissected out and subjected to western blotting analysis using antibodies against pCREB and actin as described in ‘Materials and methods' section. A representative western blot is shown for each brain region examined. (a) Hippocampus, (b) Cortex, (c) PFC, (d) NAC, (e) VTA, (f) Striatum. Data from multiple experiments (6–8 animals per group) were tabulated and presented as relative ratio of phosphorylated/actin (mean±SEM). One-way ANOVA with Tukey's test for multiple comparisons, n=6–8, *p<0.05, **p<0.01.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.
4.
Figure 1

Figure 1. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Modulation of mitogen-activated protein kinase (MAPK), calcium/calmodulin-dependent kinase II (CaMKII), GluR1, and CREB 30 min after acute morphine administration. Rats were treated with a single injection of saline or morphine (10 mg/kg, s.c.) and killed 30 min later. Brain regions were dissected out and subjected to western blotting analysis using antibodies against pMAPK, total MAPK (a), pCaMKII, total CaMKII (b), pGluR1, total GluR1 (c), pCREB, and actin (d) as described in the ‘Materials and methods' section. A representative western blot is shown for each of the proteins studied. Data from multiple experiments (4–6 animals per group) were tabulated and presented as relative ratio of phosphorylated/total forms (mean±SEM). One-way ANOVA with unpaired two-tailed t-test, n=4–6, *p<0.05; **p<0.01.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.
5.
Figure 8

Figure 8. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Effect of δ-opioid receptor (δOR) antagonist pretreatment on the induction of pGluR1 levels. The paradigm used for development of sensitization to morphine was modified in that animals were pretreated with naltriben (0.1 mg/kg, s.c.) 15 min before the administration of morphine both during sensitization and conditioning as described in ‘Materials and methods' section. Brain regions were dissected out and subjected to western blotting analysis using antibodies against phospho and total GluR1 as described in ‘Materials and methods' section. A representative western blot is shown for each brain region examined. (a) Hippocampus, (b) Cortex, (c) PFC, (d) NAC, (e) VTA, (f) Striatum. Data from multiple experiments (4–6 animals per group) were tabulated and presented as relative ratio of phosphorylated/total GluR1 levels (mean±SEM). One-way ANOVA with Tukey's test for multiple comparisons, n=6–8, *p<0.05, **p<0.01.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.
6.
Figure 5

Figure 5. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Phosphorylation of mitogen-activated protein kinase (MAPK), calcium/calmodulin-dependent kinase II (CaMKII), GluR1, and CREB in different brain regions of rats treated chronically with morphine without conditioned place preference (CPP). Rats were treated in their home cage with morphine or saline on days 1–5 and on 8 and 9 as described in ‘Materials and methods' section. Brain regions were dissected out and subjected to western blotting analysis using antibodies against pMAPK, total MAPK (a), pCaMKII, total CaMKII (b), pGluR1, total GluR1 (c), pCREB, and actin (d) as described in ‘Materials and methods' section. A representative western blot is shown for each of the proteins studied. Data from multiple experiments (4–6 animals per group) were tabulated and presented as relative ratio of phosphorylated/total forms (mean±SEM). One-way ANOVA with unpaired two-tailed t-test, n=4–6, *p<0.05, **p<0.01.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.
7.
Figure 4

Figure 4. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Conditioned place preference to morphine following a sensitization paradigm. (a) Rats (6–8 per group) were subjected to morphine sensitization and conditioning paradigm as described in ‘Materials and methods' section. Data represent the change of preference (the difference between the time spent in the drug-paired compartment on the testing day and the basal preference time for this compartment before drug treatment) (mean±SEM). Rats showed a stronger preference for the morphine-paired chamber (unpaired two-tailed t-test, n=6–8, ***p<0.01). (b–e) Phosphorylation pattern of mitogen-activated protein kinase (MAPK), calcium/calmodulin-dependent kinase II (CaMKII), GluR1, and CREB on morphine sensitization and conditioning. The brains from rats were collected after measuring conditioned place preference (CPP) and various brain regions were subjected to western blotting analysis using antibodies against pMAPK, total MAPK (b), pCaMKII, total CaMKII (c), pGluR1, total GluR1 (d), pCREB, and actin (e) as described in ‘Materials and methods' section. A representative western blot is shown for each of the proteins studied. Data from multiple experiments (6–8 animals per group) were tabulated and presented as relative ratio of phosphorylated/total forms (mean±SEM). One-way ANOVA with unpaired two-tailed t-test, n=6–8, **p<0.01.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.
8.
Figure 3

Figure 3. From: Modulation of Opiate-Related Signaling Molecules in Morphine-Dependent Conditioned Behavior: Conditioned Place Preference to Morphine Induces CREB Phosphorylation.

Conditioned place preference to morphine following a 6-day classical conditioning paradigm. (a) Rats (6–8 per group) were subjected to classical morphine conditioning paradigm as described in ‘Materials and methods' section. Data represent the change of preference (the difference between the time spent in the drug-paired compartment on the testing day and the basal preference time spent before drug treatment) (mean±SEM). Rats showed a significant preference for the morphine-paired chamber (unpaired two-tailed t-test, n=6–8, p<0.01). (b–e) Phosphorylation pattern of mitogen-activated protein kinase (MAPK), calcium/calmodulin-dependent kinase II (CaMKII), GluR1, and CREB upon classical morphine conditioning. The brains from rats were collected after measuring conditioned place preference (CPP) and various brain regions were subjected to western blotting analysis using antibodies against pMAPK, total MAPK (b), pCaMKII, total CaMKII (c), pGluR1, total GluR1 (d), pCREB, and actin (e) as described in ‘Materials and methods' section. A representative western blot is shown for each of the proteins studied. Data from multiple experiments (6–8 animals per group) were tabulated and presented as relative ratio of phosphorylated/total forms (mean±SEM). One-way ANOVA with unpaired two-tailed t-test, n=6–8, *p<0.05, **p<0.01.

José A Morón, et al. Neuropsychopharmacology. 2010 March;35(4):955-966.

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