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Results: 4

1.
FIGURE. 1

FIGURE. 1. From: TNF-induced miRNAs Regulate TNF-induced expression of E-Selectin and ICAM-1 on Human Endothelial Cells: Feedback Control of Inflammation.

TNF regulation of miRNA levels in human ECs. RNA was isolated from ECs that were treated or not for 2 or 24 h with TNF (10 ng/ml). Data are presented as the Log2 ratio of miRNA expression of average treated (2 h or 24 h) versus average untreated (0 h). miR-155, 31, 17-5p, -191, -126, -222, -20a are highlighted in black.

Yajaira Suárez, et al. J Immunol. ;184(1):21-25.
2.
FIGURE 4

FIGURE 4. From: TNF-induced miRNAs Regulate TNF-induced expression of E-Selectin and ICAM-1 on Human Endothelial Cells: Feedback Control of Inflammation.

Regulation of neutrophil adhesion by miR-31 and/or miR-17-3p. ECs transfected for 12 h with M-miR-31 and/or M-miR17-3p or CM (A) or with I-miR-31 and/or, I-miR-17-3 or CI (B) and 24 h after the transfection period treated or not with TNF. Calcein AM labeled neutrophils were added to the ECs, incubated at 37°C for 15 min. Graphs show the mean of number bound cells from triplicates ± SD of one experiment of three with similar results. *Significantly different from TNF treated control, p≤0.05.

Yajaira Suárez, et al. J Immunol. ;184(1):21-25.
3.
FIGURE 3

FIGURE 3. From: TNF-induced miRNAs Regulate TNF-induced expression of E-Selectin and ICAM-1 on Human Endothelial Cells: Feedback Control of Inflammation.

miR-17-3p regulation TNF-induced ICAM-1 expression by targeting the ICAM-1 3’UTR. COS-7 cells were co-transected with the indicated constructs and with 40 nM of M-miR-17-3p or CM (A) or with ICAM-1 3’UTR construct and 40 nM of the indicated miRNA mimics or CM (B). Data are expressed as relative luciferase activity to control samples co-transfected with an equal concentration CM (mean ± SEM of three experiments performed in duplicate). *Significantly different from cells transfected with CM and with control 3’UTR and with the indicated miRNAs, p≤0.05. C and D, Western blot analysis of ICAM-1 protein levels in ECs, (C) transfected for 12 h with M-miR-17-3p or CM or (D) with I-miR-17-3p or CI and, in both cases, cells were treated or not with TNF 24 h post-transfection. Hsp-90 was used as loading control. Tie2 served as a control protein that is not targeted by miR-17-3p. Graphs in the right show the relative total ICAM-1 protein levels compared to non-treated CM or CI-transfected controls (mean ± SEM of four experiments). *Significantly different from TNF treated control, p≤0.05.

Yajaira Suárez, et al. J Immunol. ;184(1):21-25.
4.
FIGURE. 2

FIGURE. 2. From: TNF-induced miRNAs Regulate TNF-induced expression of E-Selectin and ICAM-1 on Human Endothelial Cells: Feedback Control of Inflammation.

miR-31 regulation TNF-induced E-selectin expression by targeting the E-selectin 3’UTR. COS-7 cells were co-transected with the indicated constructs and with 40 nM of M-miR-31 or CM (A) or with SELE 3’UTR construct and 40 nM of the indicated miRNA mimics or CM (B). Data are expressed as relative luciferase activity to control samples co-transfected with an equal concentration CM (mean ± SEM of three experiments performed in duplicate). *Significantly different from cells transfected with CM and with control 3’UTR and with the indicated miRNAs, p≤0.05. C and D, Western blot analysis of E-selectin protein levels in ECs, (C) transfected for 12 h with M-miR-31 or CM or (D) with I-miR-31 or CI, in both cases 24 h post-transfection the cells were treated or not with TNF. Hsp-90 was used as loading control. VE-cadherin served as a control protein that is not targeted by miR-31. Graphs in the right show the relative total E-selectin protein levels compared to non-treated CM or CI-transfected controls (mean ± SEM of four experiments). *Significantly different from TNF treated control, p≤0.05.

Yajaira Suárez, et al. J Immunol. ;184(1):21-25.

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