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1.
Figure 7

Figure 7. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Mean (± s.e.m.) percentage change in M-50 (A) and theta-0 (B) measures of ICSS reward threshold, and maximum reinforcement rate (C), following bilateral microinjection of vehicle, quinpirole (5 μg), and quinpirole in combination with 25 μg 1-NA-spermine in ad libitum fed and food-restricted rats (n=7/group). Treatment effects are listed.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
2.
Figure 9

Figure 9. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Mean (± s.e.m.) percentage change in M-50 (A) and theta-0 (B) measures of ICSS reward threshold, and maximum reinforcement rate (C), following bilateral microinjection of vehicle, 0.02 μg and 0.1 μg AMPA in nucleus accumbens of ad libitum fed and food-restricted rats (n= 8 AL, 7 FR). Treatment effects are listed.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
3.
Figure 6

Figure 6. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Mean (± s.e.m.) percentage change in M-50 (A) and theta-0 (B) measures of ICSS reward threshold, and maximum reinforcement rate (C), following bilateral nucleus accumbens microinjection of vehicle, SKF-82958 (3 μg), and SKF-82958 in combination with 2.5 μg and 25 μg 1-NA-spermine in ad libitum fed (AL) and food-restricted (FR) rats. * Threshold-lowering effects of SKF-82958 were greater in FR than AL rats, p<.005. Additional treatment effects are listed.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
4.
Figure 5

Figure 5. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Average pre- and post-injection ICSS rate-frequency curves for ad libitum fed (n=13) and food-restricted (n=9) rats that received bilateral microinjections of SKF-82958 alone (3 μg in 0.5 μl) (A= ad libitum fed; C= food-restricted) or in combination with 1-NA-spermine (25 μg; B= ad libitum fed; D= food-restricted) in NAc medial shell. Points indicated by symbols correspond to mean theta-0 (x-axis intercept), M-50 (brain stimulation frequency supporting 50% of the maximum reinforcement rate), and maximum/asymptotic reinforcement rate.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
5.
Figure 2

Figure 2. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Effects of food restriction on GluR1 phosphorylation on Ser831 in nucleus accumbens. Subjects were injected with SKF-82958 (1.0 mg/kg, i.p.) or vehicle. Whole cell homogenates were immunoblotted with anti-phospho-Ser831 GluR1 or anti-GluR1 antibodies. Following densitometry, intensities of bands corresponding to phospho-Ser831 GluR1 for each subject were divided by the intensities of corresponding total GluR1 bands. Results (mean ± s.e.m.) are expressed in comparison to the normalized control, which was defined as the ad libitum fed group injected with vehicle. No differences were observed as a function of feeding condition or drug treatment.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
6.
Figure 4

Figure 4. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Effect of food restriction on GluR1 phosphorylation on Ser831 in nucleus accumbens. Subjects ingested 10% sucrose or had access to H2O for equal duration. Whole cell homogenates were immunoblotted with anti-phospho-Ser831 GluR1 or anti-GluR1 antibodies. Following densitometry, intensities of bands corresponding to phospho-Ser831 GluR1 for each subject were divided by the intensities of the corresponding total GluR1 bands. Results (mean ± s.e.m.) are expressed in comparison to the normalized control, defined as the ad libitum fed group with access to H2O. No differences were observed as a function of feeding condition or sucrose intake.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
7.
Figure 8

Figure 8. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Ratio of total GluR1 to tubulin in whole cell homogenates (A) of nucleus accumbens obtained from ad libitum fed and food-restricted subjects. Results are based on pooling of data from control subjects of Experiments 1 and 2 (i.e., subjects that did not receive SKF-82958 or sucrose prior to sacrifice; n=11 per group). Feeding groups displayed no difference on this measure, either within a single experiment or when pooled as above.
Ratio of total GluR1 to tubulin in the synaptosomal fraction (B) obtained from nucleus accumbens (NAc) tissue samples of ad libitum fed and food-restricted subjects that received no acute drug or ingestion treatment prior to sacrifice. N=10 per group with pooling of two NAc to obtain each data point. Feeding groups displayed no difference on this measure.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
8.
Figure 1

Figure 1. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Effects of food restriction (FR) on GluR1 phosphorylation on Ser845 in nucleus accumbens (NAc) and caudate-putamen (CPu). Subjects were injected with SKF-82958 (1.0 mg/kg, i.p.) or vehicle (n=5/group). Whole cell homogenates were immunoblotted with anti-phospho-Ser845 GluR1 or anti-GluR1 antibodies. Following densitometry, intensities of bands corresponding to phospho-Ser845 GluR1 for each subject were divided by the intensities of the corresponding total GluR1 bands to control for small differences in protein loading. Results (mean ± s.e.m.) are expressed in comparison to the normalized control, which was defined as the ad libitum fed (AL) group injected with vehicle. Results are displayed for NAc (panel “A”) and CPu (panel “B”) with representative immunoblots. * Effect of SKF-82958 greater in FR than AL group, p<.01. Additional treatment effects are listed.

K.D. Carr, et al. Neuroscience. ;165(4):1074.
9.
Figure 3

Figure 3. From: AMPA Receptor Subunit GluR1 Downstream of D-1 Dopamine Receptor Stimulation in Nucleus Accumbens Shell Mediates Increased Drug Reward Magnitude in Food-Restricted Rats.

Effects of food restriction (FR) on GluR1 phosphorylation on Ser845 in nucleus accumbens (NAc) and caudate-putamen (CPu). Subjects ingested ~12 ml of 10% sucrose (Suc) or had access to tap water (H2O) for an equal period (n=6–7/group). Whole cell homogenates were immunoblotted with anti-phospho-Ser845 GluR1 or anti-GluR1 antibodies. Following densitometry, intensities of bands corresponding to phospho-Ser845 GluR1 for each subject were divided by the intensities of corresponding total GluR1 bands. Results (mean ± s.e.m.) are expressed in comparison to the normalized control, defined as the ad libitum fed group with access to H2O. Results are displayed for NAc (panel “A”) and CPu (panel “B”) with a representative immunoblot for NAc. Sucrose increased phosphorylation of GluR1 in NAc of FR subjects (* p<.01 relative to FR/H2O and AL/Suc) and in CPu of both feeding groups.

K.D. Carr, et al. Neuroscience. ;165(4):1074.

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