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1.
Figure 3

Figure 3. From: Human-Specific Transcriptional Regulation of CNS Development Genes by FOXP2.

Visualization of one of the modules containing FOXP2 and FOXP2chimp differentially expressed genes. Five hundred pairs of genes with the greatest topological overlap are shown. Positive correlations are depicted in red and negative correlations are depicted in blue. The gene symbols for hub genes are accentuated in large bold text.

Genevieve Konopka, et al. Nature. ;462(7270):213-217.
2.
Figure 2

Figure 2. From: Human-Specific Transcriptional Regulation of CNS Development Genes by FOXP2.

FOXP2 and FOXP2chimp differentially transactivate target promoters independent of FOXP1 or FOXP4 interaction. a) Immunoblotting for FLAG or FOXP1 following immunoprecipitation with either FLAG or FOXP1 Abs. b) Mass spectrometry results from SH-SY5Y or 293T cells overexpressing FOXP2 or FOXP2chimp. The first number indicates the number of spectra and the second is the number of unique peptides. c) Cell growth analysis does not show a significant difference in proliferation between cells expressing FOXP2 or FOXP2chimp over time (P≤0.05). Error bars are ± s.e.m. (two-tailed Student’s t-test, n=3). d–e) Dual luciferase assays in 293T cells transiently transfected with promoter fragments driving luciferase and either FOXP2 or FOXP2chimp. Asterisks indicate P≤0.05 and error bars are ± s.e.m. (two-tailed Student’s t-test, n=3–6).

Genevieve Konopka, et al. Nature. ;462(7270):213-217.
3.
Figure 1

Figure 1. From: Human-Specific Transcriptional Regulation of CNS Development Genes by FOXP2.

FOXP2 and FOXP2chimp differentially regulate genes in SH-SY5Y cells. a) Schematic of human FOXP2 showing its major functional protein domains and the two amino acid changes in the mutant FOXP2chimp. b) Representative immunoblot for FLAG-tagged FOXP2 and FOXP2chimp stable overexpression in SH-SY5Y cells. c–e) Immunofluorescent staining of antibodies against FLAG epitope (green) and FOXP1 (red), and DAPI (blue) for nuclei. c) Vector cells demonstrate no FLAG expression, while both FOXP2 (d) and FOXP2chimp (e) expressing cells have FLAG-tagged FOXP2 in the cell nucleus. Scale bars are 5 microns. f) Subcellular fractionation followed by immunoblotting. g–h) Quantitative RT-PCR of genes that were differentially expressed in cells expressing FOXP2 compared to FOXP2chimp. Asterisks indicate P≤0.05 and error bars are ± s.e.m. (two-tailed Student’s t-test, n=3 or 4).

Genevieve Konopka, et al. Nature. ;462(7270):213-217.

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