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1.
Figure 4.

Figure 4. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Gene expression in control and Bcl-X conditional knockout mice. (A) Lungs from Cre+:Bcl-Xwt/wt and Cre+:Bcl-Xfl/fl mice were immunoblotted for Bcl-XL, Mc-1, Bcl-2, Bak, Bax, and actin. (B) Lungs from Cre+:Bcl-Xwt/wt and Cre+:Bcl-Xfl/fl mice were immunoblotted for proSP-C, CCSP, T1α, and PECAM. Each lane represents a different mouse, and blots are representative of five mice for each group.

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.
2.
Figure 3.

Figure 3. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Epithelial-specific recombination of mT/mG mice, using Sftpc–Cre mice. Lungs from Sftpc–Cre (A), mT/mG+/+ (B), Cre mT/mG+/− (C), and Cre+ mT/mG+/− (D) mice were stained for EGFP and DAPI. Fluorescent images for intrinsic red from the membrane targeted tdTomato gene, FITC-stained EGFP, and DAPI were obtained using confocal microscopy. Arrows indicate respiratory epithelial cells expressing EGFP, and arrowheads indicate nonrespiratory epithelial cells expressing the tdTomato gene. Bar in A = 50 μm.

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.
3.
Figure 5.

Figure 5. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Lung mechanics in control and Bcl-X conditional knockout mice. Airway resistance (A), airway elastance (B), alveolar compliance (C), tissue damping (D), and tissue elastance (E) were measured in Cre+:Bcl-Xwt/wt and Cre+:Bcl-Xfl/fl mice. Values represent mean ± SD of four control mice (Cre+:Bcl-Xwt/wt) and three knockout mice (Cre+:Bcl-Xfl/fl) per group.

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.
4.
Figure 2.

Figure 2. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Immunohistochemical staining of Bcl-X protein in Bcl-X control and knockout mice. Lungs from Cre+:Bcl-Xwt/wt (A, C, E, and G) and Cre+:Bcl-Xfl/fl (B, D, F, and H) mice were immunostained for Bcl-X protein that was detected as a brown stain, using 3,3′-diaminobenzidine. Sections were lightly counterstained with hematoxylin. Solid arrowheads denote airway epithelium, open arrowheads denote alveolar epithelium, and arrows denote vascular endothelium. Inset bar in F = 100 μm, and in H = 50 μm.

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.
5.
Figure 7.

Figure 7. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Apoptosis and edema in control and Bcl-X conditional knockout mice exposed to hyperoxia. (A) The number of TUNEL-positive cells to total number of DAPI-positive cells in Cre+:Bcl-Xwt/wt and Cre+:Bcl-Xfl/fl mice exposed to room air or hyperoxia was graphed. Values represent mean ± SD for n = 5 mice per group (*P < 0.0001 and #P = 0.02) (B) Total protein in bronchoalveolar lavage fluid of Cre+:Bcl-Xwt/wt and Cre+:Bcl-Xfl/fl mice exposed to room air or hyperoxia was quantified and graphed. Values represent mean ± SD for n = 5 mice per group (*P < 0.0001).

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.
6.
Figure 1.

Figure 1. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Genotypic analysis of Sftpc–Cre and floxed Bcl-X mice. (A) Representative RT-PCR analysis of Bcl-X isoforms detected in whole-lung RNA or type II cell RNA. (B) Depiction of floxed Bcl-X and Sftpc–Cre genes, with primers (arrowheads) used for genotyping mice. Open arrows in Bcl-X gene represent LoxP site flanking exons 1 and 2. Below the cartoon is a representative PCR analysis of genomic tail DNA, showing wild-type (wt) and floxed (fl) products of the Bcl-X and Cre genes.

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.
7.
Figure 8.

Figure 8. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Oxygen promotes 8-oxoguanine lesions in the lung. Lungs of adult mice exposed to room air (A) and lungs of adult mice exposed to hyperoxia (B) on Embryonic Day 18.5 (C, E), and Postnatal Day 2 (D, F) were stained with FITC-labeled 8-oxoguanine–binding peptide. Sections were counterstained with DAPI, and images were captured by confocal microscopy. Inset box in C and D is magnified 3-fold in E and F. Arrows indicate 8-oxoguanine–positive cells. Bar = 50 μm for AD, and 10 μm for E and F.

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.
8.
Figure 6.

Figure 6. From: Epithelial Ablation of Bcl-XL Increases Sensitivity to Oxygen without Disrupting Lung Development.

Apoptosis in control and Bcl-X conditional knockout mice exposed to hyperoxia. Cre+:Bcl-Xwt/wt (control) and Cre+:Bcl-Xfl/fl (knockout) conditional knockout mice were exposed to room air or hyperoxia for 60 hours. Representative images are of TUNEL staining in control and floxed Bcl-X mice exposed to room air (A, B) or hyperoxia (C, D) for 60 hours. Lungs of Cre+:Bcl-Xfl/fl mice exposed to hyperoxia were stained for TUNEL (red) and CCSP (E) or proSP-C (F) (green). Arrowheads indicate TUNEL-positive cells in airway and arrows indicate TUNEL-positive cells in alveoli. Dotted line in E defines the basement membrane of the airway. Inset bar = 50 μm.

Rhonda J. Staversky, et al. Am J Respir Cell Mol Biol. 2010 September;43(3):376-385.

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