We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 5

1.
Fig. 2

Fig. 2. From: Interactions of oxidosqualene cyclase (Erg7p) with 3-keto reductase (Erg27p) and other enzymes of sterol biosynthesis in yeast.

Synthesis of 3-ketosteroids. Radiochromatogram of non-saponifiable lipids extracted from cell homogenates of strain SDG115 (erg27) incubated with radiolabeled lanosterol. Lanosterol (peak a) was converted into 3-ketosteroid 1 (peak c) and 3-ketosteroid 2 (peak b).

S. Taramino, et al. Biochim Biophys Acta. ;1801(2):156.
2.
Fig. 5

Fig. 5. From: Interactions of oxidosqualene cyclase (Erg7p) with 3-keto reductase (Erg27p) and other enzymes of sterol biosynthesis in yeast.

Radiochromatogram of non-saponifiable lipids extracted from cell homogenates of the ERG7-overexpressing strain SMY8[pSM61.21] incubated with [14C]3-ketosteroid 2. 3-ketosteroid 2 (peak b) was converted into 3-hydroxyderivate (peak a), which was indistinguishable from ergosterol on TLC.

S. Taramino, et al. Biochim Biophys Acta. ;1801(2):156.
3.
Fig. 3

Fig. 3. From: Interactions of oxidosqualene cyclase (Erg7p) with 3-keto reductase (Erg27p) and other enzymes of sterol biosynthesis in yeast.

Chemical reduction of [14C]3-ketosteroid 1 with NaBH4. Radiochromatogram of the compounds resulting from reduction of 3-ketosteroid 1 with excess NaBH4 (see Exp. Section). Peak c is 3-ketosteroid 1, peak a is the β-hydroxy derivative of 3-ketosteroid 1, and peak b is the α-hydroxy derivative.

S. Taramino, et al. Biochim Biophys Acta. ;1801(2):156.
4.
Fig. 1

Fig. 1. From: Interactions of oxidosqualene cyclase (Erg7p) with 3-keto reductase (Erg27p) and other enzymes of sterol biosynthesis in yeast.

The S. cerevisiae ergosterol biosynthetic pathway from squalene to the end-product ergosterol. Compounds in bold match to the mixture of 3-ketosteroid 1, compounds in bold and underlined to the mixture of 3-ketosteroid 2. Genes in the pathway blocked in erg7 strains are designated in grey.

S. Taramino, et al. Biochim Biophys Acta. ;1801(2):156.
5.
Fig. 4

Fig. 4. From: Interactions of oxidosqualene cyclase (Erg7p) with 3-keto reductase (Erg27p) and other enzymes of sterol biosynthesis in yeast.

Incubation of homogenates from the erg7 strain SMY8 (Radiochromatogram A) and the ERG7-overexpressing strain SMY8[pSM61.21] (Radiochromatogram B) with [14C]3-ketosteroid 1. In A, the 3-ketosteroid 1 (peak b) was transformed only into the 3-hydroxy derivative (peak a), whereas in B, the 3-ketosteroid 1 (peak c) was transformed into both 3-hydroxy derivative (peak b) and ergosterol (peak a), the end-product of the pathway (see Results for details).

S. Taramino, et al. Biochim Biophys Acta. ;1801(2):156.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk