Results: 5

1.
Figure 1

Figure 1. From: Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae.

Flow chart for authentication of An. gambiae s.s. strains housed at the MR4. This chart starts from an unknown cohort of mosquitoes. Insecticide testing is always conducted on a subset. All groups are scanned morphologically for confirmation of An. gambiae features. susc. = susceptible, res. = resistant.

Elien E Wilkins, et al. BMC Biotechnol. 2009;9:91-91.
2.
Figure 5

Figure 5. From: Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae.

White gene SNP PCR assay. Gel electrophoresis of An. gambiae white gene PCR assay. Lanes (1 and 6) 1kb ladder marker, (2) MOPTI, (3) ZAN/U, (4) MALI, (5) PIMPERENA.

Elien E Wilkins, et al. BMC Biotechnol. 2009;9:91-91.
3.
Figure 4

Figure 4. From: Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae.

Combined An. gambiae species identification and mopti/savanna rDNA assay. Gel electrophoresis of An. gambiae species identification combined with Mopti - Savanna rDNA assay. Lanes: (1 and 5) 1kb ladder marker, (2) An. gambiae MOPTI, (3) An. gambiae KISUMU (Savanna), (4) An. gambiae ASEMBO (mixed Mopti and Savanna types [38].

Elien E Wilkins, et al. BMC Biotechnol. 2009;9:91-91.
4.
Figure 2

Figure 2. From: Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae.

Phenotypic characteristics in some MR4 An. gambiae colonies. Example of homochromy of wild type larvae when reared in a black-bottom pan (A) as opposed to a white-bottom pan (B); c+ or collared individual (C); black diamond trait (shown just below collar, D); red stripe (E), only on female larvae.

Elien E Wilkins, et al. BMC Biotechnol. 2009;9:91-91.
5.
Figure 3

Figure 3. From: Authentication scheme for routine verification of genetically similar laboratory colonies: a trial with Anopheles gambiae.

2La and TEP1 combined PCR assay. Gel electrophoresis of combined 2La and TEP1 assays. Lanes: (1 and 8) 1 kb ladder marker, (2) TEP1 refractory and 2L+a (L3-5), (3) TEP1 refractory/susceptible and 2L+a (4ARR), (4-5) TEP1 refractory and 2La (MOPTI), (6) TEP1 susceptible and 2L+a (ASEMBO1), (7) TEP1 refractory and 2L+a (L3-5). Not all possible combinations shown.

Elien E Wilkins, et al. BMC Biotechnol. 2009;9:91-91.

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