Results: 5

1.
Figure 5

Figure 5. Nicotine reduced the expression of epithelial markers in A/J mice.. From: Nicotine Promotes Tumor Growth and Metastasis in Mouse Models of Lung Cancer.

(A) E-Cadherin staining of A/J lung tumors induced by NNK or NNK+nicotine. (B) Quantitation of E-Cadherin intensity in tumors (C) β-Catenin staining of A/J lung tumors induced by NNK or NNK+nicotine. (D) Quantitation of membranous β-Catenin. (E) ZO-1 staining of A/J lung tumors induced by NNK or NNK+nicotine. (F) Quantitation of membranous ZO-1.

Rebecca Davis, et al. PLoS One. 2009;4(10):e7524.
2.
Figure 1

Figure 1. Nicotine promotes the growth of Line1 cells.. From: Nicotine Promotes Tumor Growth and Metastasis in Mouse Models of Lung Cancer.

(A) Nicotine (1 µM) promotes S-phase entry of serum starved Line1 cells in BrdU incorporation assays. (B) Nicotine (1 mg/kg) significantly increases Line1 tumor growth in Balb/c mice when administered by i.p. injection thrice weekly, p = 0.002, n = 10. (C) Nicotine (25 mg/kg/daily) also significantly increases Line1 tumor growth when administered by transdermal patches p = 0.019, n = 14.

Rebecca Davis, et al. PLoS One. 2009;4(10):e7524.
3.
Figure 3

Figure 3. Nicotine increases number and size of NNK induced lung tumors.. From: Nicotine Promotes Tumor Growth and Metastasis in Mouse Models of Lung Cancer.

(A) H&E staining of transverse sectioning of lungs (B) Representative scanned images of H&E stained coronal lung sections, tumors are outlined by boxes. Images were scanned at 20×magnification. (C) Nicotine increases the average number of lung tumors per mouse, p = 0.01, n = 8. (D) Nicotine increased tumor size significantly in A/J mice.

Rebecca Davis, et al. PLoS One. 2009;4(10):e7524.
4.
Figure 2

Figure 2. Nicotine increases metastatic potential.. From: Nicotine Promotes Tumor Growth and Metastasis in Mouse Models of Lung Cancer.

(A) Nicotine treated mice (1 mg/kg) displayed higher incidence of tumor recurrence following surgical removal of tumors compared to the vehicle control group, p = 0.01, n = 16. (B) Nicotine treated mice display significantly more lung metastasis from primary Line1 s.c. tumors. (C) H&E staining of lungs from vehicle and nicotine treated mice, nicotine treated mice display larger tumors as indicated by arrows. (D) Graph displaying the average total number of lung tumors per mouse in vehicle and nicotine (1 mg/kg) treated mice, p = 0.001, n = 16. (E) Graph displaying the average total number of lung tumors per mouse in control and nicotine patch (25 mg/kg) treated mice, p = 0.02, n = 16.

Rebecca Davis, et al. PLoS One. 2009;4(10):e7524.
5.
Figure 4

Figure 4. Nicotine enhances α7 nAChR subunit expression in Line1 cells.. From: Nicotine Promotes Tumor Growth and Metastasis in Mouse Models of Lung Cancer.

(A) Quiescent Line1 cells were treated with 1 µM nicotine for 18 h in the presence or absence of α-BT, an α7 nAChR subunit inhibitor. Nicotine enhances α7 expression, while α-BT reverses this. (B) Reverse-transcriptase coupled-PCR showing the expression of α7 nAChR subunits in serum starved Line1 cells treated with nicotine for 24 h. PCR for actin was used as the loading control. (C) α7 nAChR staining of A/J lung tumors induced by NNK or induced by NNK and exposed to Nicotine. Nicotine enhances the expression of this receptor subunit. (D) Quantitation of α7 nAChR expression in vehicle and nicotine treated tumors.

Rebecca Davis, et al. PLoS One. 2009;4(10):e7524.

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