Results: 2

1.
Figure 2

Figure 2. From: Automated DNA mutation detection using universal conditions direct sequencing: application to ten muscular dystrophy genes.

QIAxcel gel image CAV3 assays. Columns 1 through 11 contain PCR products from control DNA for CAV3 assays. The 15 base pair and 3000 base pair bands are reference markers injected before the PCR products are injected. Note that the 46XY control DNA that was used may be heterozygous for assay cv 08. Single bands and good forward sequence for this assay in several patients' DNA has been seen. Also there is a large CA repeat in the area which renders the reverse sequence poor. See Additional file 8 for size, concentration and gene coverage details.

Richard R Bennett, et al. BMC Genet. 2009;10:66-66.
2.
Figure 1

Figure 1. From: Automated DNA mutation detection using universal conditions direct sequencing: application to ten muscular dystrophy genes.

Polymerase trial experiments. lane 1 DNA ladder, lanes 2-4 are DMD assay #1 lane 2 polymerase C (PC), lane 3 Roche FastStart (FS), lane 4 polymerase D (PD) lanes5-7 are DMD assay #1a lane 5 PC, lane 6 FS, lane 7 PD, lanes 8-10 are DMD assay #1b lane 8 PC, lane 9 FS, lane 10 PD, lanes 11-13 are DMD assay #2 lane 11 PC, lane 12 FS, lane 13 PD, lanes 14-16 are DMD assay #4 lane 14 PC, lane 15 FS, lane 16 PD, lanes 17-19 are DMD assay #10 lane 17 PC, lane 18 FS, lane 19 PD, lanes 20-22 are FKRP assay #24 lane 20 PC, lane 21 FS, lane 22 PD, lanes 23-25 are FKRP assay #21 lane 23 PC, lane 24 FS, lane 25 PD.

Richard R Bennett, et al. BMC Genet. 2009;10:66-66.

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