Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 6

1.
FIGURE 4.

FIGURE 4. From: The Metastasis Efficiency Modifier Ribosomal RNA Processing 1 Homolog B (RRP1B) Is a Chromatin-associated Factor.

Localization of full-length RRP1B and RRP1B deletion mutants during mitosis. HeLa cells were transiently transfected with either the full-length RRP1B transcript (WT) or the amino terminus (ΔNop52), central (ΔMid) and carboxyl terminus (ΔC-term) deletion mutants. Immunofluorescence followed by confocal microscopy was performed by staining for the ectopically expressed protein (red) and double-stranded DNA (DAPI; blue). Bar, 10 μm.

Nigel P. S. Crawford, et al. J Biol Chem. 2009 October 16;284(42):28660-28673.
2.
FIGURE 3.

FIGURE 3. From: The Metastasis Efficiency Modifier Ribosomal RNA Processing 1 Homolog B (RRP1B) Is a Chromatin-associated Factor.

RRP1B interacts with various nucleosome-associated proteins. A, following TAP analysis to screen for RRP1B binding partners, co-IP was performed to confirm interactions between RRP1B and nucleosome-associated proteins. HEK293 cells were transiently transfected with either the full-length RRP1B transcript (WT) or the amino terminus (ΔNop52) or carboxyl terminus (ΔC-term) deletion mutants. Following immunoprecipitation, interactions were defined by blotting for the endogenous binding partner. B, indirect immunofluorescence was performed to confirm the interaction of RRP1B with these nucleosome binding proteins. HeLa cells were co-stained for ectopically expressed full-length RRP1B (red) and the endogenous interactor (green), and localization was confirmed using confocal microscopy. Bar, 10 μm.

Nigel P. S. Crawford, et al. J Biol Chem. 2009 October 16;284(42):28660-28673.
3.
FIGURE 2.

FIGURE 2. From: The Metastasis Efficiency Modifier Ribosomal RNA Processing 1 Homolog B (RRP1B) Is a Chromatin-associated Factor.

Characteristics of cells ectopically expressing RRP1B. A, co-IF followed by confocal microscopy was performed to define the cellular localization of RRP1B. Hemagglutinin-tagged, full-length RRP1B was ectopically expressed in HeLa cells to define its cellular localization. Cells were co-stained for ectopic RRP1B (red), either the nucleolar marker fibrillarin (FBL; top) or the nuclear envelope marker lamin B1 (LMNB1; bottom) (green), and double-stranded DNA (DAPI; blue). B, comparison of the structures of the full-length RRP1B (WT) with that of the amino terminus (ΔNop52), central (ΔMid), and carboxyl terminus (ΔC-term) deletion mutants. C, cellular localizations of the three deletion mutants in relation to that of the nuclear envelope protein, LMNB1. Bar, 10 μm.

Nigel P. S. Crawford, et al. J Biol Chem. 2009 October 16;284(42):28660-28673.
4.
FIGURE 5.

FIGURE 5. From: The Metastasis Efficiency Modifier Ribosomal RNA Processing 1 Homolog B (RRP1B) Is a Chromatin-associated Factor.

RRP1B interacts and co-localizes with a number of heterochromatin- and euchromatin-associated proteins. A, sequential nuclear extraction confirms that nucleolar RRP1B associates with DNA and the nuclear matrix. B, co-IP was used to define interactions with either HP1α or AcH4K5. HeLa cells (HP1α co-IP) or HEK293 (AcH4K5 co-IP) were transiently transfected with full-length RRP1B. Following immunoprecipitation performed with chromatin preparations, interactions were defined by blotting for the endogenous binding partner. HeLa cells were used for the HP1α co-IP due to the higher endogenous levels of this protein. C, indirect immunofluorescence was performed to confirm the interaction of RRP1B with these chromatin-associated proteins. HeLa cells were co-stained for ectopically expressed full-length RRP1B (red) and the endogenous interactor (green), and localization was confirmed using confocal microscopy. Bar, 10 μm.

Nigel P. S. Crawford, et al. J Biol Chem. 2009 October 16;284(42):28660-28673.
5.
FIGURE 6.

FIGURE 6. From: The Metastasis Efficiency Modifier Ribosomal RNA Processing 1 Homolog B (RRP1B) Is a Chromatin-associated Factor.

The effect of ectopic expression of either the wild type (1421C) or variant (1421T) RRP1B alleles on the expression of transcripts regulated by RRP1B interactors in HeLa cells. qPCR analysis was performed to determine the effect of RRP1B variant allele expression upon the transcriptional targets of TRIM28 (A) or CSDA (B). Transcript expression levels were compared between HeLa cells ectopically expressing either RRP1B allelic variant or lacZ control cultures. Significance levels were calculated by comparing relative transcript quantities of RRP1B-transfected and lacZ-transfected HeLa cells using the Mann-Whitney U test. Significant comparisons at the p < 0.05 level are marked with an asterisk.

Nigel P. S. Crawford, et al. J Biol Chem. 2009 October 16;284(42):28660-28673.
6.
FIGURE 1.

FIGURE 1. From: The Metastasis Efficiency Modifier Ribosomal RNA Processing 1 Homolog B (RRP1B) Is a Chromatin-associated Factor.

The Rrp1b activation signature predicts the risk of survival but not relapse in multiple breast cancer data sets. A, a significant difference in the overall likelihood of survival was observed in the Pawitan et al. (20) data set, with 8-year survival being 92% versus 69% for the good and poor prognosis Rrp1b signatures, respectively. B, 12-year disease-free survival was 71% versus 56% in the Ivshina et al. (22) data set for the good and poor prognosis Rrp1b signatures, respectively. C, a similar effect was observed in the Miller et al. (21) data set with 12-year survival being 90% versus 65% for the good and poor prognosis Rrp1b signatures, respectively. D, the Wang et al. (23) data set differs in that the study end point is local or distant disease recurrence rather than patient death. No survival effect was observed with Rrp1b signature gene survival in this data set.

Nigel P. S. Crawford, et al. J Biol Chem. 2009 October 16;284(42):28660-28673.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk