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Figure 1.

Figure 1. From: Combinatorial Ligand-directed Lung Targeting.

Framework for discovery and validation of organ-specific vascular markers. In the ex vivo steps, endothelial cells isolated from different organs of the H-2Kb-tsA58 mouse (termed ImmortoMouse; see Reference 51) are maintained in culture at permissive temperature. Using the in vitro phage display method BRASIL (biopanning and rapid analysis of selective interactive ligands; see Reference 40), peptides targeting these cell populations are selected, validated, and prioritized based on their binding profile. Peptide ligands are further validated by phage in vivo assays in which, after tail-vein injection, phage homing to the different vascular beds are quantified by colony count or immunostaining with anti-bacteriophage specific antibodies. The peptides targeting organ-specific vascular ligands then serve as a template for the design (in silico) and synthesis of organ targeting-agents. These designed agents can be delivered in vivo for targeted therapy applications, generation of animal models of degenerative diseases, or in vivo imaging of organ specific diseases.

Ricardo J. Giordano, et al. Proc Am Thorac Soc. 2009 August 15;6(5):411-415.

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