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Results: 3

1.
Fig. 3.

Fig. 3. From: Expression and Characterization of CYP4V2 as a Fatty Acid ?-Hydroxylase.

Effect of HET0016 (1–1000 nM) on CYP4V2-catalyzed ω-hydroxylation of lauric acid (100 μM). HET0016 is a potent inhibitor of CYP4V2, as evidenced by the IC50 value of 38 nM.

Mariko Nakano, et al. Drug Metab Dispos. 2009 November;37(11):2119-2122.
2.
Fig. 1.

Fig. 1. From: Expression and Characterization of CYP4V2 as a Fatty Acid ?-Hydroxylase.

Analysis of CYP4V2 expressed in insect cells by SDS-polyacrylamide gel electrophoresis with Coomassie staining (a), and Western blotting with an anti-His6 antibody (b). Lane 1, molecular weight markers. Lane 2, microsomes from mock-infected Sf9 cells. Lane 3, microsomes from Sf9 cells infected with baculovirus-containing CYP4V2. Lanes 2 and 3 were loaded with 10 μg of protein. c, CO difference spectrum of CYP4V2-containing insect cell microsomes demonstrating absorbance of holo-P450 at ∼450 nm.

Mariko Nakano, et al. Drug Metab Dispos. 2009 November;37(11):2119-2122.
3.
Fig. 2.

Fig. 2. From: Expression and Characterization of CYP4V2 as a Fatty Acid ?-Hydroxylase.

GC-MS analysis of lauric acid metabolites. a, bis-TMS derivatives of hydroxy lauric acid standards monitored at m/z 345, 117, 131, and 145 for 12-, 11-, 10-, and 9-hydroxy lauric acid, respectively. The derivatized internal standard (IS), 15-hydroxypentadecanoic acid, was monitored at m/z 387. b, metabolites produced from CYP4V2 microsomes in the absence of NADPH. Similar data were obtained with NADPH and mock-transfected insect cells. c, metabolites produced from CYP4V2 microsomes in the presence of NADPH. A trace amount of 11-hydroxy lauric acid was formed, but this is not evident in the chromatographic traces because the ion current intensities are normalized to the major product.

Mariko Nakano, et al. Drug Metab Dispos. 2009 November;37(11):2119-2122.

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