Results: 3

1.
FIG. 1.

FIG. 1. From: DNA-Stable Isotope Probing Integrated with Metagenomics for Retrieval of Biphenyl Dioxygenase Genes from Polychlorinated Biphenyl-Contaminated River Sediment .

[12C]- and [13C]DNA were separated by small-scaled secondary isopycnic centrifugation and quantified by quantitative PCR of 16S rRNA genes in triplicate samples. Solid circles and lines, D14H; open circles and dashed lines, D0; open triangles and dashed lines, unlabeled D14.

Woo Jun Sul, et al. Appl Environ Microbiol. 2009 September;75(17):5501-5506.
2.
FIG. 3.

FIG. 3. From: DNA-Stable Isotope Probing Integrated with Metagenomics for Retrieval of Biphenyl Dioxygenase Genes from Polychlorinated Biphenyl-Contaminated River Sediment .

Amino acid sequence alignment of large subunit of BphALB400, BphAL11E10, and BphAKF707. Only the positions that are not identical among the three BphAs are shown in the alignment. The numbers of amino acid positions are for BphALB400. The shaded areas represent conserved amino acid sequences. AA, amino acid.

Woo Jun Sul, et al. Appl Environ Microbiol. 2009 September;75(17):5501-5506.
3.
FIG. 2.

FIG. 2. From: DNA-Stable Isotope Probing Integrated with Metagenomics for Retrieval of Biphenyl Dioxygenase Genes from Polychlorinated Biphenyl-Contaminated River Sediment .

(A) Schematic diagram of gene order in clone L11E10. Following are gene product descriptions: cosL, large subunit of carbon monoxide dehydrogenase; coxM, middle subunit of carbon monoxide dehydrogenase; coxS, small subunit of carbon monoxide dehydrogenase; prpF, AcnD (the homolog of acnA)-accessory protein; acnA, aconitate hydratase; rpoE, RNA polymerase sigma 70 factor; desA, fatty acid desaturase; cfaA, cyclopropane fatty acyl phospholipid synthase; bphA, large subunit of biphenyl dioxygenase; bphE, small subunit of biphenyl dioxygenase; recJ, single-stranded DNA-specific exonuclease; rpfE, regulatory protein; greA, transcription elongation factor; and carB, large subunit of carbamoyl phosphate synthase. The order of genes rpoE-ORF3-desA-ORF4-ORF5-cfaA-ORF6-ORF7-ORF8 (gray arrows) and recJ-rpfE-greA (black arrows) in L11E10 was identical to that in six sequenced Xanthomonas genomes. (B) G+C contents of window size 500 bp. The line in the middle refers to the average G+C content of insert DNA in L11E10.

Woo Jun Sul, et al. Appl Environ Microbiol. 2009 September;75(17):5501-5506.

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