We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 3

1.
Figure 2

Figure 2. From: Efficient and Rapid identification of Candida albicans allelic status using SNP-RFLP.

Use of the diagnostic SNP-RFLP marker set confirms heterozygosity of all 32 markers in strain SC5314. The chromosome is indicated below each set of gel panels. For each digest the uncut (u) and cut (c) PCR products are shown. Order of the SNP markers corresponds to their position on the chromosome from left to right. M: molecular size marker; fragment sizes on the left are shown in kb. Precise SNP marker location and RE fragment sizes are detailed in Table 2.

Anja Forche, et al. FEMS Yeast Res. ;9(7):1061-1069.
2.
Figure 1

Figure 1. From: Efficient and Rapid identification of Candida albicans allelic status using SNP-RFLP.

Whole genome map of SNP-RFLP markers. Homolog ‘a’ is colored in black, homolog ‘b’ is colored in grey, Major repeat sequences are indicated as black filled boxes except for Chr3 (RB2 only; hashed box). The centromere is indicated as a grey oval. SNP-RFLP markers from the diagnostic set are boxed in black. Stars indicate location of individual markers. Numbers above stars indicate the unique identifier number of each marker. Centromere and MRS are not to scale.

Anja Forche, et al. FEMS Yeast Res. ;9(7):1061-1069.
3.
Figure 3

Figure 3. From: Efficient and Rapid identification of Candida albicans allelic status using SNP-RFLP.

Detection of LOH and trisomy by SNP-RFLP analysis
Each gel is labeled as in Figure 2, with SNP markers aligned from left to right along the relevant chromosome. The uncut control (u) lane is shown for each cut (c) PCR product. Colored circle cartoons illustrate the LOH events for each strain. Blue indicates allele ‘a’, pink indicates allele ‘b’. Two vertical circles represent one locus. Grey ovals represent the centromeres.
A. An LOH occurred at the left-most SNP marker on Chr1L in strain YJB10019.
B. LOH of both markers on Chr2L in strain YJB10019.
C. Whole chromosome LOH is detected as LOH of all 4 SNP markers on ChrR in strain YJB10019.
D. Skewed ratios of the two alleles of Chr2 suggest trisomy of Chr2 in CAI4-F2 and CAI4-F3 strains. Allele ‘a’ is present in 2 copies and allele ‘b’ is present in 1 copy (see also Table 2 for restriction patterns and allele assignments).
E. Skewed ratios are seen for only one of the four markers on Chr1 in strain CAI4-F3.
F. Of the two heterozygous SNPs present in both clinical strains FH1 and FH6 (markers 1 and 2) marker 2 has a skewed allelic ratio, consistent with the idea that Chr5L is trisomic in strain FH6 but not in parental strain FH1.
M, DNA size marker; u - undigested; c - cut; 1–4: SNP markers from left to right on chromosome.

Anja Forche, et al. FEMS Yeast Res. ;9(7):1061-1069.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk