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1.
Figure 6

Figure 6. Modulation of microbiota-dependent colitis directly affects tumor development.. From: Modulation of the Intestinal Microbiota Alters Colitis-Associated Colorectal Cancer Susceptibility.

A. Comparison of inflammation scores for Il10−/− mice under SPF, B. vulgatus mono-associated and germ free conditions. B. Tumor multiplicity in Il10−/− mice under SPF, B. vulgatus mono-associated and germ free conditions.

Joshua M. Uronis, et al. PLoS ONE. 2009;4(6):e6026.
2.
Figure 3

Figure 3. Il10−/− mice exhibit accelerated tumor progression.. From: Modulation of the Intestinal Microbiota Alters Colitis-Associated Colorectal Cancer Susceptibility.

A. Percent of tumor-bearing WT and Il10−/− mice with high-grade or invasive carcinoma (upper panel). Representative low-grade adenoma observed in WT mice (middle panel). Representative invasive carcinoma observed in Il10−/− mice (lower panel).

Joshua M. Uronis, et al. PLoS ONE. 2009;4(6):e6026.
3.
Figure 4

Figure 4. Il10−/− mice develop inflammation displaying active Rel A (NFkB) signaling.. From: Modulation of the Intestinal Microbiota Alters Colitis-Associated Colorectal Cancer Susceptibility.

A. Phosphorylated-Rel A immunostaining of normal Il10−/− colon tissue. B. Enlargement of Rel A positive immune infiltrate in A. C. Phosphorylated Rel A immunostaining in Il10−/− adenoma. D. Enlargement of adenoma in C. E. Rabbit IgG negative control.

Joshua M. Uronis, et al. PLoS ONE. 2009;4(6):e6026.
4.
Figure 7

Figure 7. Il10−/−; Myd88−/− mice show decreased tumor multiplicity and expression of Il12p40 and Tnfα mRNA.. From: Modulation of the Intestinal Microbiota Alters Colitis-Associated Colorectal Cancer Susceptibility.

A. Representative histology observed in Il10−/− and Il10−/−; Myd88−/− mice treated with AOM. B. Tumor multiplicity in Il10−/− and Il10−/−; Myd88−/− mice treated with AOM. C. Relative expression of Il12p40 and Tnfα mRNA in the distal colons of Il10−/− and Il10−/−; Myd88−/− mice.

Joshua M. Uronis, et al. PLoS ONE. 2009;4(6):e6026.
5.
Figure 2

Figure 2. Analysis of tumor induction and inflammation.. From: Modulation of the Intestinal Microbiota Alters Colitis-Associated Colorectal Cancer Susceptibility.

A. Representative examples of WT (left panel) and Il10−/− colons (middle and right panels) from AOM-treated mice 16 weeks after transfer to SPF conditions. B. Tumor penetrance and multiplicity in WT and Il10−/− mice (left and right panels) (p = 0.034). C. WT and Il10−/− colon inflammation scores, distal colon Il12p40 and Tnfα mRNA levels after 18–20 weeks under SPF conditions (p = 0.026).

Joshua M. Uronis, et al. PLoS ONE. 2009;4(6):e6026.
6.
Figure 5

Figure 5. Il10−/− normal colon and adenomas exhibit increased cell proliferation.. From: Modulation of the Intestinal Microbiota Alters Colitis-Associated Colorectal Cancer Susceptibility.

A. Ki67 positive cells are restricted to crypt bases in normal WT colon. Normal colons from Il10−/− mice exhibit elongated crypts with expanded Ki67 positive staining. B. Representative adenoma from Il10−/− mouse shows increased Ki67 positive staining (left panel). Rabbit IgG negative control (right panel). C. Il10−/− adenoma shows positive CTNNB1 staining.

Joshua M. Uronis, et al. PLoS ONE. 2009;4(6):e6026.
7.
Figure 1

Figure 1. Experimental timeline for AOM-induced colitis-associated colon tumorigenesis and analysis.. From: Modulation of the Intestinal Microbiota Alters Colitis-Associated Colorectal Cancer Susceptibility.

Germ free mice were transferred to SPF conditions and allowed to acclimate for 5 weeks. Mice were given AOM injections once a week for 6 weeks (black arrowheads). Development of colitis and tumor formation was monitored by colonoscopy from weeks 4 to 18 after transfer from germ free to SPF conditions (white arrowheads). Mice were sacrificed between 18 and 20 weeks and tissues processed for histological and mRNA expression analysis.

Joshua M. Uronis, et al. PLoS ONE. 2009;4(6):e6026.

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