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1.
Figure 2

Figure 2. From: Genome-wide screening of human T-cell epitopes in influenza A virus reveals a broad-spectrum of CD4+ T cell responses to internal proteins, hemagglutinins and neuraminidases.

Broad T cell responses to influenza viral proteins in PBMC from healthy adults. PBMC from four healthy adults were screened for IFN-γ responses to all influenza proteins in ELISPOT assays.

Jenny Aurielle B. Babon, et al. Hum Immunol. ;70(9):711-721.
2.
Figure 3

Figure 3. From: Genome-wide screening of human T-cell epitopes in influenza A virus reveals a broad-spectrum of CD4+ T cell responses to internal proteins, hemagglutinins and neuraminidases.

Distribution of SFC values of positive peptides in ELISPOT assays. The SFC values for each peptide that gave a positive IFN-γ response were plotted in a histogram chart to determine the frequency of IFN-γ-producing cells among PBMC of the four donors.

Jenny Aurielle B. Babon, et al. Hum Immunol. ;70(9):711-721.
3.
Figure 4

Figure 4. From: Genome-wide screening of human T-cell epitopes in influenza A virus reveals a broad-spectrum of CD4+ T cell responses to internal proteins, hemagglutinins and neuraminidases.

Flow cytometry to determine the phenotypes of IFN-γ producing cells in donor 3 PBMC. Peptide-stimulated bulk culture cells using donor 3 PBMC were used as effectors in an ICS. The peptides shown here were used to initially stimulate whole PBMC and establish the bulk culture. The final peptide concentration was 10μg/ml in all experiments. Events were gated for the live, CD3+ IFN-γ+ cells.

Jenny Aurielle B. Babon, et al. Hum Immunol. ;70(9):711-721.
4.

Figure 1. From: Genome-wide screening of human T-cell epitopes in influenza A virus reveals a broad-spectrum of CD4+ T cell responses to internal proteins, hemagglutinins and neuraminidases.

IFN-γ response of donor 1 PBMC to peptides from all influenza proteins. PBMC was first tested in ELISPOT against Set 1 peptide pools (1-33), which include the hemagglutinin (H1 HA, H3 HA and H5 HA) and neuraminidase (aN1 NA, hN1 NA and N2 NA) peptides (A) and Set 2 peptide pools (1-38), which include peptides spanning all the viral internal proteins (C). Positive pools were deconvoluted to test individual peptides. An example of such screening is shown in B and D. ELISPOT assays were performed using individual peptides in Pools 7, 10, and 11 consisting of hemagglutinin H3 HA peptides (B); and individual peptides in Pools 9, 10, 11, and 12 consisting of matrix protein 1 (M1) peptides (D). A/Wisconsin/67/2005X-161B H3N2 virus and A/New Caledonia/20/1999 IVR-66 H1N1 virus were used as positive controls for live influenza A virus infection. Peptide pools and individual peptides were tested in three replicate wells with 200,000 to 250,000 cells per well. The final concentration of peptide used in all experiments was 2μg/ml per each peptide. The dotted line indicates the cut-off SFC value for this donor (20 SFC/106).

Jenny Aurielle B. Babon, et al. Hum Immunol. ;70(9):711-721.

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