Results: 3

1.
Figure 2

Figure 2. From: A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish.

Nox/Duox activity is required for wound margin H2O2 production and leukocyte recruitment. (a) Scheme of mammalian NADPH oxidases also found in zebrafish12,13. (b) Wound margin [H2O2] ± DPI or VAS2870 (VAS), or carrier (1% DMSO) imaged 17 min pw. (c) Statistical quantification of wound margin [H2O2]. (d) Injured tail fins of mpo::GFP9 larvae ± DPI (42 min pw). Coloured lines: leukocyte tracks derived from the corresponding time-lapse movies. (e) Statistical quantification of leukocyte recruitment to wound margin. Error bars: SEM of indicated number of larvae (brackets). *** P < 0.001 (vs. control). Scale bars: 100 μm.

Philipp Niethammer, et al. Nature. ;459(7249):996-999.
2.
Figure 1

Figure 1. From: A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish.

Wound margin H2O2 production in zebrafish larvae. (a) Experimental procedure. (b) HyPer imaging in an injured zebrafish larva. [H2O2] is inferred from the YFP500/YFP420 excitation ratio of HyPer. Greyscale scaling is adjusted to improve contrast. (c) Temporal [H2O2] profile in a ∼10−30 μm broad region of interest along the wound margin. Arrival of first leukocyte at wound (solid red line) ± SD (dashed red line). (d) [H2O2] line profile normal to the wound margin. (e) Imaging of leukocyte recruitment and [H2O2] in a lysC::DsRED210 fish line. Coloured lines: superimposed leukocyte tracks. Scale bars: 100 μm.

Philipp Niethammer, et al. Nature. ;459(7249):996-999.
3.
Figure 3

Figure 3. From: A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish.

Duox activity is required for wound margin H2O2 production and leukocyte recruitment. (a) Wound margin H2O2 after morpholino mediated duox knockdown (MO1-duox) or injection of a corresponding 5-misprime morpholino (5-MP) imaged 17 min pw. Inset: RT-PCR of a duox mRNA region flanking the targeted splice site. (b) Quantification of wound margin [H2O2]. (c) Injured tail fins of mpo::GFP9 larvae injected with MO1-duox, or 5-MP (42 min pw). Coloured lines: leukocyte tracks. (d) Quantification of leukocyte recruitment. Error bars: SEM of indicated number of larvae (brackets). ** P < 0.01, *** P < 0.001 (vs. control). Scale bars: 100 μm.

Philipp Niethammer, et al. Nature. ;459(7249):996-999.

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