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1.
FIGURE 6

FIGURE 6. Follicular conjunctivitis in non-human primates. From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

These images are examples of both an uninfected naïve and an infected upper conjunctiva four weeks post-challenge. The infected conjunctiva exhibits severe hyperemia, edema and large follicles.

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.
2.
FIGURE 4

FIGURE 4. Titer and specificity of the serum ELISA antibody responses for different trachoma serovars. From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

The trachoma strain specificity of the serum antibody response was evaluated by ELISA using EBs of four different strains. (A) Serum IgG and (B) Serum IgA. ELISA titers were measured 34 days after the second boost.

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.
3.
FIGURE 7

FIGURE 7. Chlamydial shedding after ocular challenge of non-human primates. From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

The nMOMP and OVA immunized non-human primates were ocularly challenged with strain A2497. Chlamydial shedding was evaluated at weekly intervals to monitor the course of infection. The nMOMP immunized animals shed significantly fewer organisms during the first two weeks post-challenge (p =0.002-0.026, Wilcoxon rank sum test).

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.
4.
FIGURE 3

FIGURE 3. Kinetics of the serum ELISA IgG and IgA titers against serovar A EB (A2497). From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

The serum antibody responses of the immunized monkeys were measured by ELISA using formalin-fixed EBs (A2497). (A) Serum IgG and (B) Serum IgA. ELISA titers were measured approximately 30 days after the primary vaccination and the first and second boosts.

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.
5.
FIGURE 8

FIGURE 8. Gross clinical pathology after ocular challenge of non-human primates. From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

The nMOMP and OVA immunized non-human primates were ocularly challenged with strain A2497. Gross clinical pathology was evaluated at weekly intervals to monitor the course of infection. The clinical response score was determined based on the hyperemia and follicular formation of the upper conjunctiva of both eyes, with 0 indicating no disease and 12 indicating maximum disease. There was no significant difference in the gross clinical response between the two groups.

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.
6.
FIGURE 5

FIGURE 5. Titer and specificity of the serum neutralizing antibody responses for different trachoma serovars. From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

The infection neutralization capability of the serum antibodies was measured in an in vitro neutralization assay in HAK cells. (A) The kinetics of the neutralization titers were measured approximately 30 days after the primary vaccination and the first and second boosts using A2497. (B) The trachoma strain specificity of these neutralizing titers was measured 34 days after the second boost.

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.
7.
FIGURE 1

FIGURE 1. Immunization and infectious challenge schedule for nMOMP vaccinated monkeys. From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

Three cynomolgus monkeys were immunized with nMOMP three times. The immunizations were a combination of intramuscular and subcutaneous injections. Three other monkeys were immunized with OVA in a similar fashion. Approximately one month after each immunization, blood and tear samples were collected to monitor the immune response. Thirty-four days after the second boost, all animals were challenged with serovar A strain A2497. Chlamydial shedding and gross clinical response were monitored weekly after challenge.

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.
8.
FIGURE 2

FIGURE 2. Immunized monkeys recognize both trimeric and monomeric MOMP by western blot analysis. From: Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Non-Human Primates: Implication for a Trachoma Transmission Blocking Vaccine.

nMOMP was purified from C. trachomatis serovar A strain A2497 infected cells. The purity of the immunogen and its heat-labile trimeric state were evaluated by SDS-PAGE followed by Coomassie staining or immunoblotting with or without boiling. (A) Coomassie staining of nMOMP. The trimeric and the monomeric forms are indicated by arrows on the left. (B) Control immunoblot of a Serovar A specific monoclonal antibody (A-20). (C) Immunoblot using the three immunized monkey sera. (D) Immunoblot using pools of OVA and pre-bleed controls.

Laszlo Kari, et al. J Immunol. ;182(12):8063-8070.

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