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1.
Figure 1.

Figure 1. From: Effects of Fenbendazole on the Murine Humoral Immune System.

Experimental scheme for immunologic testing. T1 is after completion of the first week of treatment; T2 is after completion of the second week of treatment; T3 is after completion of the third week of treatment; T4 is after completion of the fourth week of treatment; T5, T6, and T7 are at 2, 4, and 6 wk after the fourth week of FBZ treatment. EMSA, electrophoretic mobility shift assay; LPS, lipopolysacharide.

Ana Marie Landin, et al. J Am Assoc Lab Anim Sci. 2009 May;48(3):251-257.
2.
Figure 2.

Figure 2. From: Effects of Fenbendazole on the Murine Humoral Immune System.

FBZ has no effect on proB cell expansion in both young and old mice. (A) ProB cells from untreated (white bars) or FBZ-treated (black bars) young mice were harvested, counted, and stained for membrane expression of B220 and CD43 both ex vivo and after IL7 stimulation for 7 d. Fold expansion of proB cells was calculated by dividing the percentage of proB cells after IL7 culture by the percentage found ex vivo. (B) ProB cells from untreated (white bars) or FBZ-treated (black bars) old mice were harvested, counted, and stained for membrane expression of B220, and CD43 both ex-vivo and after IL7 stimulation for 7 d. Fold expansion of proB cells was calculated as described for panel A. There were no significant differences between treated and untreated groups.

Ana Marie Landin, et al. J Am Assoc Lab Anim Sci. 2009 May;48(3):251-257.
3.
Figure 3.

Figure 3. From: Effects of Fenbendazole on the Murine Humoral Immune System.

FBZ affects E2A mRNA expression proB cells from in old but not young mice. (A) ProB cells from untreated (white bars) or FBZ-treated (black bars) young mice were stimulated with IL7 in culture for 7 d, harvested, counted, RNA-extracted, and used in RT-PCR assays. E2A:GAPDH mRNA ratios for young untreated mice were normalized to 100% (Y axis). (B) ProB cells from old mice untreated (white bars) or FBZ treated (black bars) were treated as described in panel A. Significant differences between untreated and treated groups were calculated by using 2-tailed Student t tests.

Ana Marie Landin, et al. J Am Assoc Lab Anim Sci. 2009 May;48(3):251-257.
4.
Figure 4.

Figure 4. From: Effects of Fenbendazole on the Murine Humoral Immune System.

FBZ decreases E47 protein expression in expanded proB cells from both young and old mice. E47:control (Ubc9) values for untreated young and old mice were normalized individually to 100%. (A) ProB cells from untreated (white bars) or FBZ-treated (black bars) young mice were stimulated with IL7 in culture for 7 d, harvested, counted, and used to produce nuclear extracts for Western blots. (B) ProB cells from untreated (white bars) or FBZ-treated (black bars) old mice were treated as described in panel A. Significant differences between untreated and treated groups were calculated by using 2-tailed Student t tests; there were no differences between any groups at T1 through T3 (data not shown).

Ana Marie Landin, et al. J Am Assoc Lab Anim Sci. 2009 May;48(3):251-257.
5.
Figure 7.

Figure 7. From: Effects of Fenbendazole on the Murine Humoral Immune System.

FBZ particularly decreases E2A mRNA levels in activated splenic B cells from old mice. (A) Splenic B cells (106 cells/mL) from untreated (white bars) or FBZ-treated (black bars) young mice were cultured in the presence of LPS (10 μg/mL) for 24 h. After this time, mRNA was extracted from equal numbers of cultured B cells and RT-PCR reactions performed. E2A:GAPDH mRNA ratios for young untreated mice were normalized to 100%. (B) Splenic B cells from untreated (white bars) or FBZ-treated (black bars) were treated as described in panel A. Significant differences between untreated and treated groups were calculated by using 2-tailed Student t tests.

Ana Marie Landin, et al. J Am Assoc Lab Anim Sci. 2009 May;48(3):251-257.
6.
Figure 6.

Figure 6. From: Effects of Fenbendazole on the Murine Humoral Immune System.

FBZ decreases DNA binding by E47 only in activated splenic B cells from old mice. (A) Splenic B cells (106 cells/ml) from untreated (white bars) or FBZ-treated (black bars) young mice were cultured in the presence of LPS (10 μg/mL) for 48 h. Nuclear extracts from equal numbers of cultured B cells were prepared and run in EMSA (10 μg per lane). Results for the untreated groups were normalized to 100% as compared with a nonspecific band not supershifted by the antibody. (B) Splenic B cells from untreated (white bars) or FBZ-treated (black bars) old mice were treated as described in panel A. Significant differences between untreated and treated groups were calculated by using 2-tailed Student t tests.

Ana Marie Landin, et al. J Am Assoc Lab Anim Sci. 2009 May;48(3):251-257.
7.
Figure 5.

Figure 5. From: Effects of Fenbendazole on the Murine Humoral Immune System.

FBZ treatment decreases proliferation in both young and old splenic B cells only at T4. (A) Splenic B cells (106 cells/mL) from untreated (white bars) or FBZ-treated (black bars) young mice were cultured either in the absence or presence of LPS (10 µg/mL) for 48 h. Results are expressed as stimulation indices (SI) of the untreated groups normalized to 100%. The SI is the counts per minute (cpm; mean ± SE) from triplicate cultures in the presence of stimulus divided by that from triplicate cultures in the absence of stimulus. (B) Splenic B cells from untreated (white bars) or FBZ-treated (black bars) old mice were processed as described in panel A. Significant differences between untreated and treated groups were calculated by using 2-tailed Student t tests.

Ana Marie Landin, et al. J Am Assoc Lab Anim Sci. 2009 May;48(3):251-257.

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