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1.
Figure 5

Figure 5. Current production of Geobacter sulfurreducens wild type and omcZ mutant.. From: Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells.

Current production time course of wild type and omcZ-deletion mutant. The data is a representative time course for multiple replicates of each treatment.

Kelly P. Nevin, et al. PLoS ONE. 2009;4(5):e5628.
2.
Figure 4

Figure 4. RT-PCR of genes up-regulated in microarray analyses.. From: Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells.

Fold change of pilA, omcB, omcE, omcS, omcZ and GSU1497 at different amounts of current produced compared to a soluble Fe(III) control as determined by quantitative RT-PCR.

Kelly P. Nevin, et al. PLoS ONE. 2009;4(5):e5628.
3.
Figure 6

Figure 6. Confocal scanning laser microscopy images of Geobacter sulfurreducens mutants.. From: Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells.

Confocal scanning laser microscopy images of current harvesting mutant biofilms and complemented strains . Cell stained green indicate metabolically active, cells strain red indicate metabolically inactive. A–C. slices through biofilm parallel to electrode large panel and perpendicular to electrode top and side panel; A. omcZ mutant, B. omcZ complement, C. pilA complement; D–E. 3-D projection, top view, fumarate control biofilms, D. omcZ mutant, E. pilA mutant.

Kelly P. Nevin, et al. PLoS ONE. 2009;4(5):e5628.
4.
Figure 1

Figure 1. Confocal scanning laser microscopy images of Geobacter sulfurreducens grown with different electron acceptors.. From: Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells.

Confocal scanning laser microscopy images of current harvesting and fumarate control biofilms of wild type G. sulfurreducens. Metabolically active (green) and inactive (red) cells where differentiated with a LIVE/DEAD kit based on the permeability of the cell membrane. A. 3-D projection, top view, fumarate control biofilm; B. slices through biofilm parallel to electrode large panel and perpendicular to electrode top and side panel, fumarate control biofilm; C. 3-D projection, top view, current harvesting biofilm; D. slices through biofilm parallel to electrode large panel and perpendicular to electrode top and side panel, current harvesting biofilm.

Kelly P. Nevin, et al. PLoS ONE. 2009;4(5):e5628.
5.
Figure 3

Figure 3. OmcZ sequence and identification.. From: Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells.

A. Amino acid sequence of OmcZ. The predicted cleavage site for mature OmcZ indicated by arrow. Confirmed heme-binding domains (CXXCH) are enclosed in boxes. OmcZ has 473 amino acid residues and 7 heme binding domains. B. Cytochrome content of loosely bound outer membrane protein-enriched fractions from fumarate and current-harvesting biofilms. Proteins (10 µg/lane) were separated by 12% Tris-Tricine denaturing polyacryamide gel electrophoresis and stained for heme. C. Peptides detected from two different sizes of OmcZs, fragments detected in the 50 KDa OmcZ are indicated in panel A in red; fragments detected in the 30 KDa OmcZ are indicated in panel A in green and fragments detected in both size OmcZ are indicated in blue.

Kelly P. Nevin, et al. PLoS ONE. 2009;4(5):e5628.
6.
Figure 2

Figure 2. Current production of Geobacter sulfurreducens.. From: Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells.

A. Current production time courses of wild type G. sulfurreducens grown entirely as current harvesting (arrow indicates the switch from original feed of 10 mM acetate to continual feed) and current production time course of fully grown fumarate control biofilms switched to current harvesting of wild type G. sulfurreducens. These data are representative time courses for multiple replicates of each treatment. B–C. Confocal scanning laser microscopy images of fumarate control swapped to current harvesting biofilms of G. sulfurreducens . Metabolically active (green) and inactive (red) cells where differentiated with a LIVE/DEAD kit based on the permeability of the cell membrane. B. 3-D projection, top view; C. slices through biofilm parallel to electrode large panel and perpendicular to electrode top and side panel.

Kelly P. Nevin, et al. PLoS ONE. 2009;4(5):e5628.

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