Results: 5

1.
Fig. 2

Fig. 2. MyD88-dependent cytokine induction by LT-IIb-B5 in BMDC. From: In vivo and in vitro adjuvant activities of the B subunit of Type IIb heat-labile enterotoxin (LT-IIb-B5) from Escherichia coli.

BMDC from wild-type or MyD88−/− mice were stimulated with LT-IIb-B5 or a TLR2-nonbinding mutant (S74D) (10μg/ml). Pam3Cys (100ng/ml) and LPS (100ng/ml) were used as positive controls. Induction of TNF-α (A) or IL-6 (B) production in culture supernatants was measured by ELISA. Data are means with SD (n = 3) from one of two independent experiments yielding similar results. Asterisks indicate significantly (p < 0.05) enhanced cytokine production compared to medium only.

Shuang Liang, et al. Vaccine. ;27(32):4302-4308.
2.
Fig. 3

Fig. 3. Upregulation of class II MHC and of costimulatory molecule expression in BMDC by LT-IIb-B5. From: In vivo and in vitro adjuvant activities of the B subunit of Type IIb heat-labile enterotoxin (LT-IIb-B5) from Escherichia coli.

BMDC were incubated with medium only or stimulated for 16 h with LT-IIb-B5 (10 μg/ml) and expression of the indicated surface molecules was assayed by flow cytometry. BMDC stimulated with LPS (100 ng/ml) were used as positive controls. The histograms shown are from one of five independent sets of experiments that yielded similar findings. Numbers in the histograms are mean fluorescent intensity values.

Shuang Liang, et al. Vaccine. ;27(32):4302-4308.
3.
Fig. 1

Fig. 1. Agonist dose- and cell dose-dependent cytokine induction by LT-IIb-B5 in BMDC. From: In vivo and in vitro adjuvant activities of the B subunit of Type IIb heat-labile enterotoxin (LT-IIb-B5) from Escherichia coli.

BMDC were stimulated with LT-IIb-B5 (A–D) or a TLR2-nonbinding mutant (S74D) (C–D). Pam3Cys (100ng/ml) and LPS (100ng/ml) were used as positive controls (C–D). Induction of TNF-α (A,C) or IL-6 (B,D) production in culture supernatants was measured by ELISA. A and B show agonist dose-dependent cytokine induction, whereas C and D show BMDC dose-dependent cytokine induction. Asterisks indicate significantly (p < 0.05) higher cytokine production by stimulation compared to no agonist stimulation. Data are means with SD (n = 3) from one of three independent sets of experiments yielding similar results. Asterisks indicate significantly (p < 0.05) enhanced cytokine production compared to medium-only stimulation.

Shuang Liang, et al. Vaccine. ;27(32):4302-4308.
4.
Fig. 5

Fig. 5. Mucosal adjuvanticity of the B pentameric subunit of LT-IIb holotoxin (LT-IIb-B5). From: In vivo and in vitro adjuvant activities of the B subunit of Type IIb heat-labile enterotoxin (LT-IIb-B5) from Escherichia coli.

Groups of BALB/c mice were intranasally immunized with S. mutans AgI/II, in the absence or presence of LT-IIb-B5 or intact LT-IIb adjuvant, at days 1, 15, and 29. Antibody responses (salivary IgA [A], vaginal IgA [B], serum IgA [C], and serum IgG [D]) were monitored at days 22, 36, and 50. Data are means with standard deviations (n = 6). Black circles indicate statistically significant (p < 0.05) enhancement of antibody responses compared to sham immunization, whereas asterisks show significant (p < 0.05) enhancement of antibody responses compared to AgI/II (“Ag”) alone.

Shuang Liang, et al. Vaccine. ;27(32):4302-4308.
5.
Fig. 4

Fig. 4. Induction of CD4+ T cell proliferation by LT-IIb-B5-stimulated BMDC. From: In vivo and in vitro adjuvant activities of the B subunit of Type IIb heat-labile enterotoxin (LT-IIb-B5) from Escherichia coli.

Mouse BMDC were stimulated or not for 16 h with wild-type (WT) or mutant (S74D) LT-IIb-B5 (10μg/ml). After washing three times with culture medium, the stimulated BMDC were co-cultured with CFSE-stained CD4+ T cells in the presence of suboptimal concentration of anti-CD3. After staining with PE-Cy5-anti-CD4, proliferation of gated CD4+ T cells was analyzed by FACS (A). In a similar co-culture experiment, CD4+ T cell proliferation was assessed using the BrdU assay, where the results are presented as % increase in BrdU incorporation over medium-only stimulation (B). Pam3Cys (200ng/ml) and LPS (100ng/ml) were used as positive controls (B). (A) Shown are representative histograms from three independent experiments. (B) Data are means with SD (n = 3) from one of three independent experiments yielding similar findings. The asterisk indicates significant (p < 0.05) differences between wild-type LT-IIb-B5 and S74D mutant.

Shuang Liang, et al. Vaccine. ;27(32):4302-4308.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk