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Results: 7

1.
Fig. (2)

Fig. (2). From: HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

Cocaine and benztropine induce phosphorylation of Erk 1/2, MARCKS and MLC in BMVEC’s.

Milan Fiala, et al. Open Virol J. 2008;2:24-31.
2.
Fig. (4)

Fig. (4). From: HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

Ultrastructural features of monocyte migration through the BMVEC model of the blood-brain barrier. Compared to the untreated cells (a), cocaine-treated BMVEC’s (b) display cytoplasmic vacuolization (TEM, 10,000 x).

Milan Fiala, et al. Open Virol J. 2008;2:24-31.
3.
Fig. (5)

Fig. (5). From: HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

Perivascular infiltration by macrophages in HAD brain tissues. (a) Minimal perivascular macrophage infiltration in patient 1 (IHC with CD68 antibody). (b) Robust macrophage infiltration of vessels in an AIDS patient dying in late 1980’s.

Milan Fiala, et al. Open Virol J. 2008;2:24-31.
4.
Fig. (1)

Fig. (1). From: HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

Cocaine disrupts BMVEC junctions. Confluent BMVEC monolayers were treated with the indicated concentration of cocaine for 1h; the cells were then washed, fixed, stained with phalloidin-ALEXA 594 and photographed by Hamamatsu camera on the Olympus Bmax microscope (40x). The arrows indicate gaps between BMVEC’s.

Milan Fiala, et al. Open Virol J. 2008;2:24-31.
5.
Fig. (3)

Fig. (3). From: HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

Ultrastructural features of monocyte migration through the BMVEC model of the blood-brain barrier. (A) Endothelial layer becomes thick above the pores through which the monocytes are migrating (1,000x section of the BBB model with migrating monocytes). (B) Monocytes migrate between endothelial cells and through the pore (TEM, 10,000 x).

Milan Fiala, et al. Open Virol J. 2008;2:24-31.
6.
Fig. (7)

Fig. (7). From: HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

(p24, Nef) Immunohistochemical demonstration of HIV-1 antigens in frontal lobe neurons of the patient #5 with HAD (the results of staining in patients #1-4 were comparable) (a). Positive neuronal staining with anti p24 (ISF2) (40x, inset 100x). (b) Positive neuronal staining with anti p24 (AG 3.0) (100x). (c) Positive neuronal staining with anti-Nef (AE6). (d) Positive neuronal staining with anti-Nef (EH1).

Milan Fiala, et al. Open Virol J. 2008;2:24-31.
7.
Fig.(6).

Fig.(6). From: HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

(gp120). Immunohistochemical demonstration of HIV-1 antigens in frontal lobe neurons of the patient #5 with HAD (the results of staining in patients #1-4 were comparable). (a) Positive neuronal gp120 staining (anti-gp120 (ID6) (brown) and anti-MAP2 (red), 40x). (b) Positive gp120 staining of neurons and negative staining of astrocytes (anti-gp120 (ID6) (brown) and anti-GFAP (red), 40x). (c) Positive gp120 staining of neurons (anti-gp120 (ID6) absorbed with the negative phage 2.13, 100x). (d) Negative gp120 staining of neurons (anti-gp120 (ID6) absorbed with the positive phage 3.3, 100x).

Milan Fiala, et al. Open Virol J. 2008;2:24-31.

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