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1.
Figure 2

Figure 2. From: The mir-34 microRNA is required for the DNA damage response in vivo in C. elegans and in vitro in human breast cancer cells.

miR-34 expression in C. elegans post-irradiation. Similar increase in miR-34 expression was detected in both wild-type N2 animals and cep-1/p53 mutants at 3 hours post-irradiation. Error bars show s.d. An asterisk indicates statistical significance.

M Kato, et al. Oncogene. ;28(25):2419-2424.
2.
Figure 3

Figure 3. From: The mir-34 microRNA is required for the DNA damage response in vivo in C. elegans and in vitro in human breast cancer cells.

mir-34 loss-of-function mutants have altered cell survival post-irradiation. (a) Loss of miR-34 expression in mir-34(gk437) was confirmed by northern blot. (b) mir-34 loss-of-function mutants were sensitive to non-apoptotic cell death. (c) mir-34 mutants are resistant to apoptotic cell death. (d) mir-34(gk437); cep-1(gk138) double mutants were significantly more radiosensitive to non-apoptotic cell death than either of the single mutant. Animals were irradiated with 200 Gy. Similar results were obtained from radiation treatment with 400 Gy.

M Kato, et al. Oncogene. ;28(25):2419-2424.
3.
Figure 1

Figure 1. From: The mir-34 microRNA is required for the DNA damage response in vivo in C. elegans and in vitro in human breast cancer cells.

Sequence alignment of mir-34 family MicroRNAs (miRNAs) and the expression of mir-34 in C. elegans (a) Highly conserved regions are highlighted by black (100%) and gray (>50%). The seed region, GGCAGU, is lined. Abbreviations for each organism are shown in Supplementary information 5. (b) Developmental northern blot of mir-34 and gfp signals of mir-34gfp. Magnification and exposure time for detecting gfp are shown in each image. (c) mir-34gfp expression in vulval cells in the adult stage.

M Kato, et al. Oncogene. ;28(25):2419-2424.
4.
Figure 4

Figure 4. From: The mir-34 microRNA is required for the DNA damage response in vivo in C. elegans and in vitro in human breast cancer cells.

Post-irradiation breast cancer cell survival is impacted by mir-34 alterations. (a) miR-34a levels were lower in triple negative (TN) and mesenchymal breast cancer cell lines compared with normal epithelial lines and Her-2+ lines. Details are shown in Supplementary information 4. (b) Radiation sensitivity of breast cancer cell lines. The cells treated with radiation (2.5 Gy) were plated in clonogenic assays compared with non-irradiated samples. Error bars represent s.d. (c) Breast cell lines HMEC, MCF10A and MDAMB-231 did not undergo apoptotic cell death post-irradiation in contrast to what was seen in Jurkat cells. The fold-increase in CPP32 activity post-irradiation was compared with the un-irradiated controls. (d) miR-34a levels alter cell survival in MDA-MB-231 breast cancer cell lines in response to increasing radiation doses.

M Kato, et al. Oncogene. ;28(25):2419-2424.

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