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1.
Fig. 1

Fig. 1. From: Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study.

Human basophil gating with the BD-FACScanto flow cytometer. In a leukocytes are immunologically gated in the CD45dim-lymphocyte area and electronically captured as cellular events in HLADRnon-expressing/CD123bright two-parameter plot to distinguish them from monocytes (Q1), plasmacytoid dendritic cells (Q2) and lymphocytes (Q3). Dot plotsb and c show CD63/CD203c expression in resting and anti-IgE-activated human basophils, respectively. The cloud of resting CD203clow expressing/CD63negative cells (area C in b) shifts to the right upon activation (c), but only a small percentage of cells express CD63 (area B in c)

Salvatore Chirumbolo, et al. Inflamm Res. 2009 November;58(11):755-764.
2.
Fig. 2

Fig. 2. From: Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study.

Percentage inhibition of anti-IgE-activated CD203c expression in human basophils treated with high histamine dilutions (a) or with high water dilutions (b). Mean ± SEM percentages from five experiments in (a) and from five experiments in (b) were calculated after subtracting the constitutive fluorescence of resting cells from the fluorescence of anti-IgE-activated cells, as described in “Materials and methods”. *P < 0.05, **P < 0.01 (two-tailed Wilcoxon test for paired data, comparing histamine or water dilutions with the mean of untreated controls in each experiment). CD203c MFI values of the uninhibited activation were 2576.6 ± 543.3 SEM in the histamine dilution experiments (a) and 4142.1 ± 348.4 SEM in the water dilution experiments (b)

Salvatore Chirumbolo, et al. Inflamm Res. 2009 November;58(11):755-764.

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