Results: 4

1.
Fig. 1.

Fig. 1. From: High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infections.

Naive CD4+ T cell are the major source of infectious virus circulating in the blood during the acute infection of rhesus monkeys with SHIVDH12R. The frequencies of productively-infected naïve or memory cells collected on day 10 PI from 3 SHIVDH12R-infected animals were determined by cocultivation with MT4 cells for 14 days as described ().

Yoshiaki Nishimura, et al. Proc Natl Acad Sci U S A. 2009 May 12;106(19):8015-8020.
2.
Fig. 4.

Fig. 4. From: High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infections.

Quantitation of integrated viral DNA in naïve or memory CD4+ T cells collected from acutely SIV or SHIV-infected macaques. (A) Genomic DNA from 3D8 cells was serially diluted 2-fold, in triplicate, starting with 500 cell DNA equivalents and analyzed by Alu-LTR PCR. (B) Genomic DNAs, purified from sorted naïve or memory CD4+ T cells from SHIVDH12R-infected macaque 95P005 on day 10 PI, were serially diluted, and integrated proviral DNA was measured by Alu-LTR PCR. (C) The frequencies of naïve or memory CD4+ T cells carrying integrated SIV or SHIV DNA were determined by Alu-LTR PCR and normalized to the values obtained with the 3D8 cell line.

Yoshiaki Nishimura, et al. Proc Natl Acad Sci U S A. 2009 May 12;106(19):8015-8020.
3.
Fig. 2.

Fig. 2. From: High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infections.

Peripheral blood CD4+ T cell profiles and viral nucleic acid levels during the acute SIV and SHIV infections of macaques. (A) Circulating CD4+ T cell numbers and plasma viral RNA loads were measured in a SIV (CN30)-infected and a SHIVDH12R (WGG)-infected rhesus monkey at the indicated times. (B) PBMC samples, collected on days 7 and 10 PI from macaques CN30 and WGG, were stained with anti-CD3, CD4, CD28, and CD95 mAbs to distinguish naïve from memory CD4+ T cells and sorted by FACS for determinations of the cell-associated viral DNA copies per 105 cells by DNA PCR.

Yoshiaki Nishimura, et al. Proc Natl Acad Sci U S A. 2009 May 12;106(19):8015-8020.
4.
Fig. 3.

Fig. 3. From: High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infections.

Resting CD4+ T cells from acutely SIV and SHIV-infected macaques continue to release progeny virions ex vivo. (A) Nonactivated CD4+ T cells were purified by negative FACS sorting from PBMCs collected on day 7 from macaque CN30 (SIVmac239) or day 10 from macaque WGG (SHIVDH12R) as described in Methods. The FACS profiles of CD4+ HLA-DR+ cells from each monkey are shown. (B) Purified resting CD4+ T cells were treated with Pronase to eliminate any surface-bound virions and cultured for 4 days in the absence of any stimulation in RPMI medium 1640 supplemented with 15% autologous serum. Culture supernatants were collected daily, filtered, and spinoculated on MT-4 T cells, in duplicate, to amplify low levels of released progeny virions. Virus production was assessed by measuring the 32P_RT activity released into the medium of duplicate MT-4 cultures on day 14 PI by autoradiography.

Yoshiaki Nishimura, et al. Proc Natl Acad Sci U S A. 2009 May 12;106(19):8015-8020.

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