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1.
FIG. 5.

FIG. 5. From: Contribution of Lipoproteins and Lipoprotein Processing to Endocarditis Virulence in Streptococcus sanguinis .

Map of the ssaB locus and complementation constructs. Symbols: open horizontal arrows, genes; filled horizontal arrows, putative ssaACB promoters; flags, transposon insertion sites in the ssaB mutant. See Materials and Methods and Results for further details.

Sankar Das, et al. J Bacteriol. 2009 July;191(13):4166-4179.
2.
FIG. 4.

FIG. 4. From: Contribution of Lipoproteins and Lipoprotein Processing to Endocarditis Virulence in Streptococcus sanguinis .

Lipoprotein processing in S. sanguinis.
 (A) [3H]palmitate labeling of SK36 and selected isogenic mutants. The migration of molecular mass standards is indicated to the left. Arrow, 33-kDa band present in SK36—presumably SsaB, which is approximately 33 kDa in its mature form— but not in the ssaB mutant. (B) Western blot analysis of the same strains shown in panel A using an antiserum reactive against SsaB.

Sankar Das, et al. J Bacteriol. 2009 July;191(13):4166-4179.
3.
FIG. 1.

FIG. 1. From: Contribution of Lipoproteins and Lipoprotein Processing to Endocarditis Virulence in Streptococcus sanguinis .

Predicted S. sanguinis SK36 lipoprotein and neighboring ABC transport component genes. The number (SSA-) of each gene is indicated. Adjacent genes are connected by black lines. Nonlipoprotein ABC transport genes are depicted only when separated from a lipoprotein gene and from one another by no more than a single non-ABC component gene. Predicted component classifications are indicated by symbol shape; COG categories are indicated by color. Orientation and relative positions of transposon insertions are indicated by flags above the gene symbols; allelic exchanges are indicated by orange boxes below the symbols. Genes and intergenic regions are not drawn to scale. ATPB, ATP-binding.

Sankar Das, et al. J Bacteriol. 2009 July;191(13):4166-4179.
4.
FIG. 2.

FIG. 2. From: Contribution of Lipoproteins and Lipoprotein Processing to Endocarditis Virulence in Streptococcus sanguinis .

Virulence assessment of lipoprotein mutants by STM. Hybridization signals from probes derived from the inoculum pool or from vegetations recovered from infected rabbits are shown on the left. The number (SSA-) or name of the mutated gene corresponding to the signature tag used as a hybridization target for each spot is indicated in the keys on the right. Mutants associated with weak signals in all the vegetation blots shown are indicated with solid lines surrounding the signals on the blots and the mutant designations in the keys. Gray outlines of designations in the keys indicate mutants that produced weak vegetation signals in experiments not shown here. Dashed outlines of designations in the keys highlight two mutants possessing independently derived, differently tagged transposon insertions within the SSA_1382 gene. Neg., negative control target derived from a signature-tagged transposon not used for mutant creation. (Top) Pool 1 experiment, testing 40 mutants; (bottom) pool 2 experiment, testing 17 mutants.

Sankar Das, et al. J Bacteriol. 2009 July;191(13):4166-4179.
5.
FIG. 6.

FIG. 6. From: Contribution of Lipoproteins and Lipoprotein Processing to Endocarditis Virulence in Streptococcus sanguinis .

Analysis of the ssaB-complemented strains JFP57 and JFP58. (A) In vivo CI analyses of the ssaB mutant and complemented strains. Mean CI values are indicated in parentheses. Symbols are used as described in the legend to Fig. 3. JFP58 values were obtained from two separate experiments using three or four animals each, whereas the values for the other two strains were obtained from a single experiment employing two or four animals each. (B) Semiquantitative Western blot analysis of SsaB expression in JFP57 and JFP58. The indicated amount of each lysate was loaded onto an SDS-PAGE gel and analyzed by Western blotting. Replicate blots performed on different days with the same and independently derived lysates produced similar results.

Sankar Das, et al. J Bacteriol. 2009 July;191(13):4166-4179.
6.
FIG. 3.

FIG. 3. From: Contribution of Lipoproteins and Lipoprotein Processing to Endocarditis Virulence in Streptococcus sanguinis .

In vivo and in vitro CI analyses of selected lipoprotein mutants. The gene mutated in each test strain is indicated by the number (SSA-) or gene name at the bottom of each graph. The dashed line denotes a CI value of 1, indicating equal competitiveness. Each symbol indicates the CI value derived from a single animal or bacterial culture; solid horizontal lines indicate geometric mean values. (A) In vivo CI analysis. Mean CI values from five to six animals tested in two separate experiments each are indicated in parentheses. Those values that were significantly different from 1 are indicated, as follows: *, P of <0.05; **, P of <0.01; ***, P of <0.005; ****, P of <0.001. (B) In vivo CI analysis versus in vitro CI analysis. Open symbols, in vivo CI values taken from values shown Fig. 3A; closed symbols, in vitro CI values obtained from three to four separate cultures. In vitro CI values that were significantly different from 1 are indicated as in panel A.

Sankar Das, et al. J Bacteriol. 2009 July;191(13):4166-4179.

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