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Results: 3

1.
Fig. 2.

Fig. 2. From: From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.

(a) Distribution of gene length in the B. subtilis 168 genome. The absence of any overrepresentation of short CDSs supports the view that most if not all gene sequences predicted in the present annotation are authentic. (b) Correspondence analysis of the proteome of B. subtilis. Proteins in the proteome can be separated into two well-identified classes. The green cloud corresponds to proteins that are integral inner-membrane proteins (IIMPs). Note that the IIMP cloud is driven by the opposition between charged amino acids (D, E and K) and hydrophobic ones (F, L, M, W).

Valérie Barbe, et al. Microbiology. 2009 June;155(Pt 6):1758-1775.
2.
Fig. 1.

Fig. 1. From: From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.

Comparison between the previously published sequence of strain 168 and the strain resequenced without cloning. SNPs and indels are as indicated, as well as the uneven distribution of G+C nucleotides in the sequence. Under the line representing the genome are displayed the positions of the different regions attributed to the various members of the sequencing consortium. It can be seen that the amount of variation is dependent on the sequencing group, not on the nucleotide composition of the genome. In some regions there is precious little variation, compared with the present sequence, despite the fact that the techniques used between 10 and 20 years ago were very different from those used today.

Valérie Barbe, et al. Microbiology. 2009 June;155(Pt 6):1758-1775.
3.
Fig. 3.

Fig. 3. From: From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.

Circular representation of the B. subtilis 168 genome for several specific genome features. Circles display the following, from the inside out. (1) GC skew (G+C/G−C using a 1 kb sliding window). (2) GC deviation (mean GC content in a 1 kb window − overall mean GC). Red areas indicate that deviation is higher than 1.5 standard deviation. (3) tRNA (dark green) and rDNA (blue). (4) Location of genomic regions with specific features differentiating them from the average sequence. Boxes coloured in light blue indicate regions of phage origin. The nonsymmetrical distribution (right and left halves of the circle) is to be emphasized. (5) Scale. (6, 7, 8) Genes having a presumed orthologue in other Bacillus species (B. licheniformis, B. amyloliquefaciens and B. pumilus respectively).

Valérie Barbe, et al. Microbiology. 2009 June;155(Pt 6):1758-1775.

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