We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 3

1.
Figure 3

Figure 3. Reduced pathogenicity of passaged SHIVmn229.. From: Complexity of the Inoculum Determines the Rate of Reversion of SIV Gag CD8 T Cell Mutant Virus and Outcome of Infection.

(A) Comparison of mean (±SEM) viral load of 21 animals infected with the same original SHIVmn229 stock (89% EM, 11% WT) to four animals inoculated with passaged SHIVmn229 isolates containing less WT virus (4% or 0.3%). (B) Comparison of individual peak viral loads. (C) Comparison of individual set point viral loads.

Liyen Loh, et al. PLoS Pathog. 2009 April;5(4):e1000378.
2.
Figure 2

Figure 2. Dependence of reversion dynamics on the percentage of WT in the inoculum.. From: Complexity of the Inoculum Determines the Rate of Reversion of SIV Gag CD8 T Cell Mutant Virus and Outcome of Infection.

(A) The time needed to reach 50% WT in total viral load depends on the fraction of WT in the inoculum, and starts to increase rapidly with the decrease in initial percentage below approximately 10%. (B) Dependence of reversion rate at 50% WT on the fraction of WT in the inoculum. In (A) and (B): full circle symbols, experimental data; open circles, obtained by extrapolation; line, results of the model for rWrM = 3×10−4 µL/cell/day. (C) The observed reversion rate is proportional to the average target cell number (Equation 3). The dashed line represents target cells in time. The red and the green full lines show how % WT in total viral load grows if it is initially 10% or 0.25%, respectively. If WT does not reach 50% before target cells are depleted, then it will take much longer to overtake EM. (D) Experimentally observed CD4+ T cell levels and % WT in the SHIVmn229-infected animal H20 (with initial 11% WT at KP9) and (E) in animal 6255, infected with the passaged SHIVmn229 with 0.34% WT at KP9 conform to the theoretical pattern in (C).

Liyen Loh, et al. PLoS Pathog. 2009 April;5(4):e1000378.
3.
Figure 1

Figure 1. Reversion to WT for different viruses and percentages of WT in the inocula.. From: Complexity of the Inoculum Determines the Rate of Reversion of SIV Gag CD8 T Cell Mutant Virus and Outcome of Infection.

(A–F) Shows WT (squares) and EM (triangles) plasma viral loads over time by qRT-PCR from individual pigtail macaques inoculated with different viruses over 11 weeks. (A) SHIVmn229 stock with 11.2% WT virus at KP9 CD8 T cell epitope (four animals) (B) In vivo passage of SHIVSF162P3 with 50% WT virus at AF9 CD8 T cell epitope (2 animals). (C) In vivo passage of SHIVmn229 with 4.0% WT virus at KP9 (2 animals). (D) In vivo passage of SHIVmn229 with 0.34% WT virus at KP9 (two animals). (E) Mix of SIVmac239 molecular clones containing 10% WT virus and 90% K165R EM virus. (F) Pure SIVmac239 molecular clone of 100% K165R EM virus (0% WT, 3 animals). (G) Mean (±SEM) of WT (upper panels) or EM (lower panels) viral loads of groups of animals given the same virus. Animals administered mixes of EM and WT virus with ≥10% WT have similar WT and EM viral loads and are grouped together (left panels) in comparison to animals administered viruses with <10% WT content (right panels). The first 10 days are shaded to indicate the differences in WT virus expansion between the two types of viruses.

Liyen Loh, et al. PLoS Pathog. 2009 April;5(4):e1000378.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk