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Results: 5

1.
Figure 4

Figure 4. Endoglin expression in the hippocampal DG. From: Effect of chronic morphine on the dentate gyrus neurogenic microenvironment.

(A) Low-power image shows that endoglin staining is present throughout the hippocampus and in the SGZ where the majority of proliferating BrdU-IR cells reside. Scale bar=100 um. (B–C) Confocal images show close proximity of BrdU-IR cells to endoglin-IR vessels. Scale bar=10 um.

Amy A. Arguello, et al. Neuroscience. ;159(3):1003-1010.
2.
Figure 3

Figure 3. Chronic morphine dynamically alters levels of VEGF in the DG. From: Effect of chronic morphine on the dentate gyrus neurogenic microenvironment.

(A, B) After 24 hrs of morphine, DG protein levels of VEGF and VEGFR2 are unchanged as measured via immunoblotting. (C, D) After 96 hrs, VEGF protein levels are increased whereas receptor levels remain unchanged. At all time points sham: n=5–6, morphine: n=4–8, **p<0.01.

Amy A. Arguello, et al. Neuroscience. ;159(3):1003-1010.
3.
Figure 2

Figure 2. Chronic morphine does not alter DG levels of BDNF or IL1β. From: Effect of chronic morphine on the dentate gyrus neurogenic microenvironment.

To determine if the microenvironment of the hippocampus was altered by chronic morphine, DG-enriched extracts were used to look for changes in protein levels via immunoblotting. (A–D) After 24 hrs of morphine exposure, dentate levels of IL1β, BDNF and their respective receptors, IL1R1 and TrkB remained unchanged. (E–H) After 96 hrs of morphine exposure, dentate levels of IL1β and BDNF and their respective receptors, IL1R1 and TrkB remained unchanged. At all time points sham: n=4–8, morphine: n=5–8.

Amy A. Arguello, et al. Neuroscience. ;159(3):1003-1010.
4.
Figure 1

Figure 1. Chronic morphine inhibits the total population of SGZ proliferating cells. From: Effect of chronic morphine on the dentate gyrus neurogenic microenvironment.

(A) C57BL/6J mice were implanted with s.c. sham or morphine pellets at 0 and 48 hrs and killed at 24 or 96 hrs via decapitation. (B) The number of Ki67-IR cells in the SGZ was not changed after 24 hrs. (C) The number of Ki67-IR cells was significantly decreased after 96 hrs of morphine exposure. (D) Representative IHC demonstrated decreased numbers of Ki67-IR cells in the SGZ. At all time points sham: n=4, morphine: n=4. Scale bar=100 μm, **p<0.01.

Amy A. Arguello, et al. Neuroscience. ;159(3):1003-1010.
5.
Figure 5

Figure 5. Chronic morphine-induced increase in VEGF correlates with altered DG neurovasculature. From: Effect of chronic morphine on the dentate gyrus neurogenic microenvironment.

(A)After 24 hrs of morphine exposure, various properties of endoglin-IR cells including vessel number, area and perimeter remain unchanged. (B) After 96 hrs of morphine exposure, the number of endoglin-IR vessels between sham and morphine groups did not differ. However the area and perimeter of endoglin-IR vessels are increased. (C) The proximity of BrdU-IR cells to endoglin-IR vessels, does not differ between sham and morphine treated groups. Proximity was measured as “touch”, touching or overlapping the vessel; “near”, within 10 μm of vessel, or “far”, beyond 10μm of vessel. For all time points sham: n=4–5, morphine: n=4–5, **p<0.01.

Amy A. Arguello, et al. Neuroscience. ;159(3):1003-1010.

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