Display Settings:

Items per page
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

Results: 9

1.
FIG. 9.

FIG. 9. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

Large nerve trunks are accessible for injections. (A) Motor neuron pool labeled by injection of HRP–ChTxB into the cervical VIII nerve. (B) Enlarged view of an area in (A). (C) Motor neuron pool labeled by injection into the saphenous nerve in lumbar spinal cord. (D) Motor neurons labeled by injection into the cervical V nerve.

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
2.
FIG. 1.

FIG. 1. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

RNAi therapeutic strategies: (1) siRNA may be delivered directly to the CNS to silence disease genes; (2) Pol III constructs synthesizing shRNA; or (3) Pol II constructs synthesizing miRNA can be placed in viral vectors and delivered into the CNS cells for long-term silencing of disease genes. See text for description of the different strategies and the RNAi mechanism.

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
3.
FIG. 2.

FIG. 2. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

A schematic illustration of different methods of administering viral vectors for delivering RNAi to spinal motor neurons: (A) direct spinal cord injection, (B) muscle injection, and (C) nerve injection. Different colors mark the different motor neuron pools that innervate different muscles. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
4.
FIG. 6.

FIG. 6. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

The effect of RAd injection on the sciatic nerve. (A) Hematoxylin and Eosin (H&E) stained section from the injected segment of the sciatic nerve. (B) A section from the uninjected side. (C) Rotorod test of G93A animals before and after injection of RAd to both sciatic nerves. “inj”, injected group; “n-op”, nonoperated group. The date at which the injected group was operated on was day 0. One test was skipped after the injection to avoid possible aggravation of the operation wounds. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
5.
FIG. 4.

FIG. 4. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

EGFP expression in spinal motor neurons by retrograde transport following sciatic nerve injection of AAV2. (A) Three weeks after injection of AAV2/CMV-EGFP. (B) A large magnification view of the boxed area in (A). Notice that besides the brightly labeled neurons, there are also many weakly labeled motor neurons in the ventral horn area. Similar strongly and weakly labeled EGFP-positive axons can be observed in the sciatic nerve: (C) a segment above the injection site, (D) a segment at the injection site, (E) a segment below the injection site. (F) A sciatic nerve segment taken from the uninjected sciatic nerve from the same animal.

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
6.
FIG. 8.

FIG. 8. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

AAV2-delivered RNAi has no effect on the disease progression in SOD1G93A mice. (A) Female SOD1G93A mice were divided into two groups: one injected with AAV2 expressing shRNA against SOD1 (n = 23) and EGFP, and the other was untreated (n = 21). The AAV2 was injected into the sciatic nerve in mice at ages from 43 days to 109 days. This group survived for 139 ± 12 days. The untreated group survived for 138 ± 12 days. (B) The correlation between the AAV2-injection age and survival was not statistically significant (p = 0.2), indicating the age of AAV2 administration did not affect survival. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
7.
FIG. 5.

FIG. 5. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

RAd-delivered RNAi can knockdown mutant SOD1 level in motor neurons. (A) The shRNA- and GFP-expression cassette that is placed in RAd. U6 and CMV promoters direct synthesis of shRNA against SOD1 and EGFP, respectively. “T” indicates the Pol III transcription termination sequence. “pA” indicates the poly A signal. (B) Robust expression of EGFP in motor neurons 7 days after injection of the RAd/U6-hSOD1hp/CMV-EGFP into the right sciatic nerve. Red represents SOD1 staining. (C and D) The majority of the EGFP expressing neurons (arrows) have lowered SOD1 staining. Motor neurons that do not express EGFP (arrowheads) have high SOD1 staining. (F–H) A control mouse injected with RAd/U6-SCRAMBLEhp/CMV-EGFP shows EGFP expression in motor neurons (arrows) but no reduced levels of SOD1. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
8.
FIG. 3.

FIG. 3. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

Comparison of motor neuron expression of EGFP between injection of RAd into gastrocnemius muscle and into sciatic nerve. Fewer motor neurons express express EGFP in mouse spinal cord 7 days after injection of RAd (1.25 × 109 particles) into gastrocnemius muscle (A) compared with the injection of the sciatic nerve (B). Notice the levels of EGFP in individual motor neurons are also lower in the muscle-injected spinal cord, compared with the nerve-injected spinal cord. By the same exposure, in the nerve-injected spinal cord the motor neurons show bright fluorescence and visible neurites, whereas in the muscle-injected spinal cord the motor neurons are dimly labeled and the neurites are not visible. (C) In another experiment, wide motor neuron expression of EGFP is observed 5 days after injection of 108 PFU into sciatic nerve. (D) High magnification view of labeled motor neurons in (C). The images are horizontal longitudinal sections of the spinal cord. In (C) and (D), only the injected sides are shown. Arrows point to the boards of lateral spinal cord.

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.
9.
FIG. 7.

FIG. 7. From: Nerve Injection of Viral Vectors Efficiently Transfers Transgenes into Motor Neurons and Delivers RNAi Therapy Against ALS.

RAd-delivered RNAi slows disease progression and extends survival of SOD1G93A mice. (A) Six pairs of female SOD1G93A littermates were divided into two groups. One was injected with RAd expressing shRNA against SOD1 and EGFP and the other was injected with RAd expressing EGFP alone. The animals were all injected at 94 days of age (arrow) and weighed before and after the injection. The average weight changes are plotted. Error bars represent standard error. We compared the weight and its changes over time (including times before and after viral injection) between the two groups using two-way ANOVA. There was no significant difference between the weights of the two groups (F = 1.6; p = 0.21). However, there was a significant difference in the weight changes over time during aging between the two groups (F = 1.8; p = 0.04). There was no significant interaction between the weight of the mice and the weight changes over time (F = 1.5; p = 0.09). (B) Animals injected with RAd expressing the shRNA lived significantly longer than those injected with the control RAd (Wilcoxon rank sum test, p = 0.022). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).

Rui Wu, et al. Antioxid Redox Signal. 2009 July;11(7):1523-1534.

Display Settings:

Items per page

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk