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1.
Figure 2

Figure 2. CD4+ T cell count prior to rhIL-7 treatment predicts the magnitude of CD4+ T cell increase after rhIL-7 treatment. . From: Enhanced T cell recovery in HIV-1-infected adults through IL-7 treatment.

The linear correlation between CD4+ T cell count before treatment at 0 d and 12 wk relative to rhIL-7 treatment initiation at each dose is shown. Overall regression using a Spearman test showed a significant correlation between CD4+ T cell counts at entry CD4+ T cell responses in both groups and in the global population of IL-7–treated patients.

Yves Levy, et al. J Clin Invest. 2009 April 1;119(4):997-1007.
2.
Figure 1

Figure 1. Effects of rhIL-7 therapy on peripheral blood lymphocyte subsets.. From: Enhanced T cell recovery in HIV-1-infected adults through IL-7 treatment.

The absolute lymphocyte count from complete blood counts and flow cytometry–based frequency were used to determine evolution of (A) absolute counts of CD3+ T cells, CD3+CD4+ T cells, and CD3+CD8+ T cells; (B) absolute counts of mature B cells and percentages of pro-B and transitional B cells; (C) absolute counts of NK and NKT cells; and (D) fold increase from baseline of CD4+ and CD8+ T cells expressing CD127. Mean ± SEM for each cohort is shown. *P < 0.05 versus 0 d; Wilcoxon test.

Yves Levy, et al. J Clin Invest. 2009 April 1;119(4):997-1007.
3.
Figure 4

Figure 4. rhIL-7 therapy is not associated with T cell activation, but increases T cell cycling.. From: Enhanced T cell recovery in HIV-1-infected adults through IL-7 treatment.

Analyses were performed in patients treated with the 10-μg/kg dose of rhIL-7. (A and B) Percent cells in CD4+ (A) and CD8+ (B) T cell subpopulations expressing Ki67 via flow cytometry. Mean ± SEM for each T cell subset is shown. Ki67 expression significantly increased at 14 d. (C) Percent CD4+ and CD8+ activated T cells (HLA-DR+CD38+). Error bars denote SD. *P versus 0 d; Wilcoxon test.

Yves Levy, et al. J Clin Invest. 2009 April 1;119(4):997-1007.
4.
Figure 3

Figure 3. rhIL-7 therapy induces a preferential increase of naive and central memory CD4+ and CD8+ T cells. . From: Enhanced T cell recovery in HIV-1-infected adults through IL-7 treatment.

(A) Representative flow cytometry analysis of CD4+ and CD8+ T cell populations from a single representative subject treated at the 3-μg/kg dose. Numbers within the plots indicate the percent of each T cell population: naive (N; CD45RA+CD27+), central memory (CM; CD45RACD27+), effector memory and effector (EM+E; CD45RACD27), terminally differentiated effector (TE; CD45RA+CD27), and RTE (CD45RA+CD31hi). (B) Time evolution of CD4+ and CD8+ T cell subsets. Mean ± SEM for each cohort is shown. *P < 0.05 versus 0 d; Wilcoxon test.

Yves Levy, et al. J Clin Invest. 2009 April 1;119(4):997-1007.
5.
Figure 5

Figure 5. rhIL-7 expanded T cells respond to polyclonal and antigenic stimulation.. From: Enhanced T cell recovery in HIV-1-infected adults through IL-7 treatment.

PBMCs were collected at the indicated time points, cryopreserved, and then thawed before analysis. (A) Proliferation in response to TCR stimulation (anti-CD3 plus anti-CD28) was measured by [3H] incorporation. Similar proliferation levels were observed at each time point. Error bars denote SD. (B) Both CD4+ and CD8+ T cells produced IFN-γ and IL-2 in response to SEB at levels comparable to baseline 28 d and 12 wk after rhIL-7 therapy. Analyses were performed in patients treated with 10 μg/kg rhIL-7. Error bars denote SD. (C) rhIL-7 therapy increased the capacity of CD4+ T cells to respond to HIV Gag and CMV antigens by secreting IFN-γ and/or IL-2. All 7 patients treated with 10 μg/kg rhIL-7 were analyzed.

Yves Levy, et al. J Clin Invest. 2009 April 1;119(4):997-1007.

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