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1.
FIGURE 2.

FIGURE 2. From: Occurrence of Mitochondria-targeted Late Embryogenesis Abundant (LEA) Gene in Animals Increases Organelle Resistance to Water Stress.

Human hepatoma cells (HepG2/C3A) transfected either with a vector encoding a chimeric protein composed of the leader sequence from AfrLEA3m plus GFP (frames Aa–Ad) or with an expression vector encoding only GFP (Ba–Bd). Co-staining with Mitotracker Red highlights the mitochondrial network (Ab and Bb; red color). Green and red fluorescence are co-localized in cells transfected with the chimeric protein (Ac) but not in cells transfected with GFP-only (Bc), where GFP remains in the cytoplasm. Mitotracker Red fluorescence is not found in the nucleus (Ac and Bc; blue indicates the nucleus). Co-localization is quantitatively supported by the scatter plot of red versus green channels in Ad, but substantial separation of green and red florescence is confirmed in Bd.

Michael A. Menze, et al. J Biol Chem. 2009 April 17;284(16):10714-10719.
2.
FIGURE 1.

FIGURE 1. From: Occurrence of Mitochondria-targeted Late Embryogenesis Abundant (LEA) Gene in Animals Increases Organelle Resistance to Water Stress.

Bioinformatic features of AfrLEA3m and its mRNA expression. A, full-length deduced amino acid sequence for AfrLEA3m from embryos of A. franciscana. The 29-amino acid N-terminal leader sequence is highlighted in red. B and C, repetitive amino acid motifs for AfrLEA3m. D, alignment of amino acids 130–140 to a historical 11-mer repeat seen for some group 3 LEA proteins. E, Kyte and Doolittle (47) hydropathy plots for the deduced protein AfrLEA3m indicates strong hydrophilicity (shown by values below zero). A more hydrophobic N-terminal region of about 30 amino acids is indicative of a subcellular targeting sequence. The position of the repetitive motifs were found with the software programs RADAR (Ra, Rb) and TRUST (Ta, Tb), and the group 3 LEA motifs are labeled. F, mRNA expression profile for the gene Afrlea3m from A. franciscana embryos. LEA mRNA is maintained 9–11-fold higher in the two desiccation-tolerant embryonic stages when compared with the desiccation-intolerant nauplius larva. Double asterisks indicate that the paired means are statistically different (t test, p < 0.05).

Michael A. Menze, et al. J Biol Chem. 2009 April 17;284(16):10714-10719.

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