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1.
Figure 9

Figure 9. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

Zeta potential distribution of PSS-stabilized GNRs at pH 8.8, in 100 mM NaCl. The trace in the figure is based on the average of three measurements.

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
2.
Figure 4

Figure 4. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

MTT cytotoxicity profile of PSS-coated GNRs using KB cells, before and after exchange with unadulterated PSS. (■) PSS-coated GNRs contaminated with CTAB (prior to PSS exchange); (□) “CTAB-free” GNRs after three times exchange and redispersion with 70-kDa PSS (4.4 μg/mL/O.D.; PSS/GNR weight ratio of 0.12). Lines drawn to guide the eye.

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
3.
Figure 5

Figure 5. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

Absorption spectroscopy of PSS and PSS-GNRs. (A) Linear absorption range of PSS (free polyelectrolyte; slope=0.053). (B) PSS-GNRs purified by membrane ultrafiltration (---); GNRs after centrifugation (6000 g, 5 min) and redispersion in deionized water (—); supernatant containing PSS stripped from GNRs (???).

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
4.
Figure 7

Figure 7. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

(A, B) Representative TEM images of PSS-stabilized GNRs; (C, D) histogram of GNR length and width distribution (71.5 ± 10.7 nm and 28.5 ± 7.3 nm, respectively; N=426). Less than 1% of the observed particles were irregularly shaped (marked with white arrows in B).

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
5.
Figure 3

Figure 3. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

MTT and LDH assays establishing the viability and membrane integrity, respectively, of LLC-PK1 cells after incubation over 48 h with the following: 45 μg/mL GNR stock solution; 100-kDa retentate (after resuspension in fresh media); 100-kDa filtrate. Percentage values are normalized with respect to a media-only control.

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
6.
Figure 8

Figure 8. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

Intensity-averaged size distribution plots of PSS-stabilized GNRs in PBS, at 35 μg/mL with backscattering optics (green trace) and 90° collection optics (orange trace), and at 3.5 μg/mL with backscattering optics (red trace). Peaks below 10 nm are due to rotational diffusion and have been omitted from size distribution analysis. Traces are based on a minimum of 12 measurements per sample.

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
7.
Figure 2

Figure 2. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

Cytotoxicity of (A) CTAB-stabilized GNR dispersions and (B) GNR dispersions treated with 70-kDa PSS and subjected by ultrafiltration (aged for 11 weeks), based on a MTT viability assay after a 24-h exposure (pH 7.4): (■) LLC-PK1 cells; (□) HepG2 cells; (▲) KB cells. All MTT viability data are normalized with respect to a media-only control. Lines drawn to guide the eye.

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
8.
Figure 6

Figure 6. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

(A) Dispersion stability of GNRs in PBS (pH 7.4), following centrifugation (6000 g) and 3× exchange with 70-kDa PSS at 4.4 μg/mL/O.D. (PSS/GNR = 0.12), then dispersed at different PSS/GNR weight ratios: (□) no PSS added; (■) PSS/GNR = 0.06; (▲) PSS/GNR = 0.15; (△) PSS/GNR = 0.37; (•) PSS/GNR = 0.98. (B) GNR dispersion stability in PBS following 3× exchange with 70-kDa PSS (4.4 μg/mL/O.D.) and redispersion in Tween 20 at a final concentration of 4 mM (ca. 5 μg/mL). Lines drawn to guide the eye.

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.
9.
Figure 1

Figure 1. From: Detoxification of Gold Nanorods By Treatment With Polystyrenesulfonate.

(A) PSS-coated GNR dispersions over a range of pH values (8.2, 9.3, 11, 12.5, 13.5), after 24 h at room temperature. Samples were prepared by diluting concentrated GNR suspensions with buffer in a 10:1 ratio. (B) Stability of dialyzed GNRs dispersed in PBS solution (pH 7.4) over 24 h, using different peptizing agents: (■) 70-kDa PSS; (□) 3.4-kDa PSS; (○) 100-kDa DSS (weight ratio of peptizing agent to GNR=0.94). Lines drawn to guide the eye.

Alexei P. Leonov, et al. ACS Nano. ;2(12):2481-2488.

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