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Results: 4

1.
Fig. 1.

Fig. 1. From: Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.

Bioinformatic analysis of 5′-flanking region of AQP2 gene. (A) Sequence conservation analysis for 1,000 bp of 5′-flanking region of AQP2 gene (http://genome.ucsc.edu). Conserved regions are centered 513 and 224 bp upstream from transcription start site. (B) Identification of conserved TR-binding element motifs in 1,000 bp of 5′-flanking region of AQP2 gene based on conserved sequence among 5 species (Genomatix).

Ming-Jiun Yu, et al. Proc Natl Acad Sci U S A. 2009 February 17;106(7):2441-2446.
2.
Fig. 4.

Fig. 4. From: Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.

Ets family transcriptional regulators transactivate the mouse proximal AQP2 promoter. Luciferase activity was measured in LLC-PK1 cells cotransfected with the AQP2-Luc reporter and either pTarget (empty vector) or Elf3, Elf5, or Ehf and stimulated with 10−7 M dDAVP and 400 μM IBMX (+dDAVP/IBMX) or vehicle (-dDAVP/IBMX). Data represent mean ± SE. (n = 12 per condition).

Ming-Jiun Yu, et al. Proc Natl Acad Sci U S A. 2009 February 17;106(7):2441-2446.
3.
Fig. 3.

Fig. 3. From: Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.

Characterization of mpkCCD clone 11. (A) AQP2 protein abundance increases after introduction of 0.1 nM dDAVP to basolateral medium. Asterisk indicates a significant difference relative to time 0 (no dDAVP). (B) Time course of phosphorylation changes of AQP2 protein in clone 11 cells based on immunoblotting with phosphospecific antibodies to 4 different phosphorylation sites in the COOH-tail of AQP2.

Ming-Jiun Yu, et al. Proc Natl Acad Sci U S A. 2009 February 17;106(7):2441-2446.
4.
Fig. 2.

Fig. 2. From: Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.

Cloning of mpkCCD-derived cell lines expressing AQP2 at various levels. (A) Confocal immunofluorescence image showing original mpkCCDc14 cells grown in presence of 0.1 nM dDAVP and immunolabeled with AQP2 antibody. (B) Laser-scanning cytometry reveals that the distribution of AQP2 immunofluorescence is biphasic indicating the presence of a heterogeneous cell population. (C) AQP2 immunoblot of homogenates from original mpkCCDc14 cells (O) and 5 clonal lines. Note broad range of AQP2 protein abundance among clonal lines.

Ming-Jiun Yu, et al. Proc Natl Acad Sci U S A. 2009 February 17;106(7):2441-2446.

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